A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I
We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) comple...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2011-10-01
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| Series: | Sensors |
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| Online Access: | http://www.mdpi.com/1424-8220/11/11/10187/ |
| _version_ | 1798024427757109248 |
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| author | Gang Liu Lihua Wang Jingwen Chen Shuzhen Ren Dun Pan Quanwen Liu Hui Xu Shuli Gao Min Ding |
| author_facet | Gang Liu Lihua Wang Jingwen Chen Shuzhen Ren Dun Pan Quanwen Liu Hui Xu Shuli Gao Min Ding |
| author_sort | Gang Liu |
| collection | DOAJ |
| description | We here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+. |
| first_indexed | 2024-04-11T18:02:25Z |
| format | Article |
| id | doaj.art-c12bafe0945c4c9ca7097811a8a84a50 |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-04-11T18:02:25Z |
| publishDate | 2011-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-c12bafe0945c4c9ca7097811a8a84a502022-12-22T04:10:26ZengMDPI AGSensors1424-82202011-10-011111101871019610.3390/s111110187A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green IGang LiuLihua WangJingwen ChenShuzhen RenDun PanQuanwen LiuHui XuShuli GaoMin DingWe here report a rapid, sensitive, selective and label-free fluorescence detection method for cysteine (Cys). The conformation of mercury-specific DNA (MSD) changes from a random coil form to a hairpin structure in the presence of Hg2+ due to the formation of a thymine-Hg2+-thymine (T-Hg2+-T) complex. Cys can selectively coordinate with Hg2+ and extract it from the thymine-Hg2+-thymine complex. The hairpin structure dehybridizes and the fluorescence intensity of Sybr Green I (SG) decreases upon addition of Cys because SG efficiently discriminates mercury-specific DNA and mercury-specific DNA/Hg2+ complex. The detection can be finished within 5 min with high sensitivity and selectivity. In addition, we can obtain variable dynamic ranges for Cys by changing the concentration of MSD/Hg2+.http://www.mdpi.com/1424-8220/11/11/10187/cysteinecompetition assaymercury-specific DNAHg2+Sybr Green I |
| spellingShingle | Gang Liu Lihua Wang Jingwen Chen Shuzhen Ren Dun Pan Quanwen Liu Hui Xu Shuli Gao Min Ding A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I Sensors cysteine competition assay mercury-specific DNA Hg2+ Sybr Green I |
| title | A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I |
| title_full | A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I |
| title_fullStr | A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I |
| title_full_unstemmed | A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I |
| title_short | A Highly Sensitive and Selective Competition Assay for the Detection of Cysteine Using Mercury-Specific DNA, Hg2+ and Sybr Green I |
| title_sort | highly sensitive and selective competition assay for the detection of cysteine using mercury specific dna hg2 and sybr green i |
| topic | cysteine competition assay mercury-specific DNA Hg2+ Sybr Green I |
| url | http://www.mdpi.com/1424-8220/11/11/10187/ |
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