Isolation, in vitro culture, and characterization of black crappie, Pomoxis nigromaculatus and white crappie, P. annularis ovarian tissue primary cells
Abstract Investigations were conducted to develop a general protocol for the isolation and in vitro culture of ovary‐derived cells from black crappie, Pomoxis nigromaculatus, and white crappie, P. annularis. Five digestive enzymes: 500 U/mL collagenase type I, 500 U/mL collagenase type IV, 0.05% try...
Main Authors: | , , , , |
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Format: | Article |
Language: | English |
Published: |
Wiley
2023-12-01
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Series: | Journal of the World Aquaculture Society |
Subjects: | |
Online Access: | https://doi.org/10.1111/jwas.12981 |