Isolation, in vitro culture, and characterization of black crappie, Pomoxis nigromaculatus and white crappie, P. annularis ovarian tissue primary cells

Abstract Investigations were conducted to develop a general protocol for the isolation and in vitro culture of ovary‐derived cells from black crappie, Pomoxis nigromaculatus, and white crappie, P. annularis. Five digestive enzymes: 500 U/mL collagenase type I, 500 U/mL collagenase type IV, 0.05% try...

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Bibliographic Details
Main Authors: Sujan Bhattarai, Jacob H. Jones, Nilima N. Renukdas, Anita M. Kelly, Dayan A. Perera
Format: Article
Language:English
Published: Wiley 2023-12-01
Series:Journal of the World Aquaculture Society
Subjects:
Online Access:https://doi.org/10.1111/jwas.12981