Using Gene Editing to Establish a Safeguard System for Pluripotent Stem-Cell-Based Therapies

Using Gene Editing to Establish a Safeguard System for Pluripotent Stem-Cell-Based Therapies

Summary: A major challenge in using human pluripotent stem cells (hPSCs) in therapy is the risk of teratoma formation due to contaminating undifferentiated stem cells. We used CRISPR-Cas9 for in-frame insertion of a suicide gene, iC9, into the endogenous SOX2 locus in human embryonic stem cell (ESC)...

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Bibliographic Details
Main Authors: Youjun Wu, Tammy Chang, Yan Long, He Huang, Fouad Kandeel, Jiing-Kuan Yee
Format: Article
Language:English
Published: Elsevier 2019-12-01
Series:iScience
Online Access:http://www.sciencedirect.com/science/article/pii/S2589004219304894

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http://www.sciencedirect.com/science/article/pii/S2589004219304894

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