3′IsomiR Species Composition Affects Reliable Quantification of miRNA/isomiR Variants by Poly(A) RT-qPCR: Impact on Small RNA-Seq Profiling Validation

3′IsomiR Species Composition Affects Reliable Quantification of miRNA/isomiR Variants by Poly(A) RT-qPCR: Impact on Small RNA-Seq Profiling Validation

Small RNA-sequencing (small RNA-seq) has revealed the presence of small RNA-naturally occurring variants such as microRNA (miRNA) isoforms or isomiRs. Due to their small size and the sequence similarity among miRNA isoforms, their validation by RT-qPCR is challenging. We previously identified two mi...

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Bibliographic Details
Main Authors: Adriana Ferre, Lucía Santiago, José Francisco Sánchez-Herrero, Olga López-Rodrigo, Josvany Sánchez-Curbelo, Lauro Sumoy, Lluís Bassas, Sara Larriba
Format: Article
Language:English
Published: MDPI AG 2023-10-01
Series:International Journal of Molecular Sciences
Subjects:
miRNA isoforms
isomiRs
poly(A)-RT-qPCR
selective amplification
small RNAseq validation strategy
Online Access:https://www.mdpi.com/1422-0067/24/20/15436

Internet

https://www.mdpi.com/1422-0067/24/20/15436

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