An easy method for quantification of anaerobic and microaerobic gene expression with fluorescent reporter proteins
Fluorescent proteins, such as green fluorescent proteins, are invaluable tools for detecting and quantifying gene expression in high-throughput reporter gene assays. However, they introduce significant inaccuracies in studies involving microaerobiosis or anaerobiosis, as oxygen is required for the m...
Main Authors: | , |
---|---|
Format: | Article |
Language: | English |
Published: |
Taylor & Francis Group
2023-12-01
|
Series: | BioTechniques |
Subjects: | |
Online Access: | https://www.future-science.com/doi/10.2144/btn-2023-0064 |