Sperm retrieval techniques for assisted reproduction
Different surgical methods such as PESA, MESA, TESA, TESE and micro-TESE have been developed to retrieve spermatozoa from either the epididymis or the testis according to the type of azoospermia, i.e., obstructive or non-obstructive. Laboratory techniques are used to remove contaminants, cellular de...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
Sociedade Brasileira de Urologia
2011-10-01
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| Series: | International Brazilian Journal of Urology |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382011000500002 |
| _version_ | 1811270140675751936 |
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| author | Sandro C. Esteves Ricardo Miyaoka Ashok Agarwal |
| author_facet | Sandro C. Esteves Ricardo Miyaoka Ashok Agarwal |
| author_sort | Sandro C. Esteves |
| collection | DOAJ |
| description | Different surgical methods such as PESA, MESA, TESA, TESE and micro-TESE have been developed to retrieve spermatozoa from either the epididymis or the testis according to the type of azoospermia, i.e., obstructive or non-obstructive. Laboratory techniques are used to remove contaminants, cellular debris, and red blood cells following collection of the epididymal fluid or testicular tissue. Surgically-retrieved spermatozoa may be used for intracytoplasmic sperm injection (ICSI) and/or cryopreservation. In this article, we review the surgical procedures for retrieving spermatozoa from both the epididymis and the testicle and provide technical details of the commonly used methods. A critical analysis of the advantages and limitations of the current surgical methods to retrieve sperm from males with obstructive and non-obstructive azoospermia is presented along with an overview of the laboratory techniques routinely used to process surgically-retrieved sperm. Lastly, we summarize the results from the current literature of sperm retrieval, as well as the clinical outcome of ICSI in the clinical scenario of obstructive and non-obstructive azoospermia. |
| first_indexed | 2024-04-12T21:55:37Z |
| format | Article |
| id | doaj.art-c18cbb454bfc47f7b51627af9c356b45 |
| institution | Directory Open Access Journal |
| issn | 1677-5538 1677-6119 |
| language | English |
| last_indexed | 2024-04-12T21:55:37Z |
| publishDate | 2011-10-01 |
| publisher | Sociedade Brasileira de Urologia |
| record_format | Article |
| series | International Brazilian Journal of Urology |
| spelling | doaj.art-c18cbb454bfc47f7b51627af9c356b452022-12-22T03:15:20ZengSociedade Brasileira de UrologiaInternational Brazilian Journal of Urology1677-55381677-61192011-10-0137557058310.1590/S1677-55382011000500002Sperm retrieval techniques for assisted reproductionSandro C. EstevesRicardo MiyaokaAshok AgarwalDifferent surgical methods such as PESA, MESA, TESA, TESE and micro-TESE have been developed to retrieve spermatozoa from either the epididymis or the testis according to the type of azoospermia, i.e., obstructive or non-obstructive. Laboratory techniques are used to remove contaminants, cellular debris, and red blood cells following collection of the epididymal fluid or testicular tissue. Surgically-retrieved spermatozoa may be used for intracytoplasmic sperm injection (ICSI) and/or cryopreservation. In this article, we review the surgical procedures for retrieving spermatozoa from both the epididymis and the testicle and provide technical details of the commonly used methods. A critical analysis of the advantages and limitations of the current surgical methods to retrieve sperm from males with obstructive and non-obstructive azoospermia is presented along with an overview of the laboratory techniques routinely used to process surgically-retrieved sperm. Lastly, we summarize the results from the current literature of sperm retrieval, as well as the clinical outcome of ICSI in the clinical scenario of obstructive and non-obstructive azoospermia.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382011000500002infertilitymaleazoospermiasperm retrievalreproductive techniquesassistedreview |
| spellingShingle | Sandro C. Esteves Ricardo Miyaoka Ashok Agarwal Sperm retrieval techniques for assisted reproduction International Brazilian Journal of Urology infertility male azoospermia sperm retrieval reproductive techniques assisted review |
| title | Sperm retrieval techniques for assisted reproduction |
| title_full | Sperm retrieval techniques for assisted reproduction |
| title_fullStr | Sperm retrieval techniques for assisted reproduction |
| title_full_unstemmed | Sperm retrieval techniques for assisted reproduction |
| title_short | Sperm retrieval techniques for assisted reproduction |
| title_sort | sperm retrieval techniques for assisted reproduction |
| topic | infertility male azoospermia sperm retrieval reproductive techniques assisted review |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-55382011000500002 |
| work_keys_str_mv | AT sandrocesteves spermretrievaltechniquesforassistedreproduction AT ricardomiyaoka spermretrievaltechniquesforassistedreproduction AT ashokagarwal spermretrievaltechniquesforassistedreproduction |