Comparison of two muscle fibre staining techniques and their relation to pork quality traits

This study compared two histochemical staining methods of muscle fibres and evaluated their relationship with the meat quality traits of two high-value porcine muscles. Immunohistochemical (IHC) and adenosine triphosphatase (ATPase) staining was used to assess the cross-sectional area and proportion...

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Main Authors: Nicole Lebedová, Tersia Needham, Jaroslav Čítek, Monika Okrouhlá, Kateřina Zadinová, Kamila Pokorná, Roman Stupka
Format: Article
Language:English
Published: Czech Academy of Agricultural Sciences 2020-06-01
Series:Czech Journal of Animal Science
Subjects:
Online Access:https://cjas.agriculturejournals.cz/artkey/cjs-202006-0001_comparison-of-x202f-two-muscle-fibre-staining-techniques-and-their-relation-to-x202f-pork-quality-traits.php
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author Nicole Lebedová
Tersia Needham
Jaroslav Čítek
Monika Okrouhlá
Kateřina Zadinová
Kamila Pokorná
Roman Stupka
author_facet Nicole Lebedová
Tersia Needham
Jaroslav Čítek
Monika Okrouhlá
Kateřina Zadinová
Kamila Pokorná
Roman Stupka
author_sort Nicole Lebedová
collection DOAJ
description This study compared two histochemical staining methods of muscle fibres and evaluated their relationship with the meat quality traits of two high-value porcine muscles. Immunohistochemical (IHC) and adenosine triphosphatase (ATPase) staining was used to assess the cross-sectional area and proportion of fibre-types I, IIa, IIx and IIb in the samples of longissimus lumborum (LL) and psoas major (PM) muscles collected one-hour post-mortem from 25 crossbred pigs [Large WhiteSire × (Landrace × Large WhiteDam)] at an average age of 152 days. Muscles differed in all fibre parameters, except the proportion and relative area of type IIx fibres. The LL muscle exhibited greater fibre cross-sectional areas of all fibre types, higher proportions of type IIb/IIB, and lower proportions of I and IIa fibres than the PM muscle in both staining techniques. These two muscles also differed marginally in moisture, crude protein and intramuscular fat content. The PM muscle showed a low correlation between fibre types and chemical composition, but the LL muscle showed moderate correlations between fibre CSA and area composition for moisture and ash content. After IHC staining, an increase in LL eye muscle area and drip loss were correlated with lower proportions of type I fibres, while a greater proportion of type IIx fibres resulted in increased LL eye muscle area and moisture content. Furthermore, a higher CSA of all fibre types in the LL decreased redness (a*) and moisture content of the muscle. Results showed that IHC is more appropriate than ATPase staining for the assessment of relationships between muscle fibre parameters and meat quality traits in pigs.
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spelling doaj.art-c1a121b620b44c5e879d498483f9a9022023-02-23T03:33:45ZengCzech Academy of Agricultural SciencesCzech Journal of Animal Science1212-18191805-93092020-06-0165619320410.17221/23/2020-CJAScjs-202006-0001Comparison of two muscle fibre staining techniques and their relation to pork quality traitsNicole Lebedová0Tersia Needham1Jaroslav Čítek2Monika Okrouhlá3Kateřina Zadinová4Kamila Pokorná5Roman Stupka6Department of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Science and Food Processing, Faculty of Tropical AgriSciences, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicDepartment of Animal Science, Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences Prague, Prague, Czech RepublicThis study compared two histochemical staining methods of muscle fibres and evaluated their relationship with the meat quality traits of two high-value porcine muscles. Immunohistochemical (IHC) and adenosine triphosphatase (ATPase) staining was used to assess the cross-sectional area and proportion of fibre-types I, IIa, IIx and IIb in the samples of longissimus lumborum (LL) and psoas major (PM) muscles collected one-hour post-mortem from 25 crossbred pigs [Large WhiteSire × (Landrace × Large WhiteDam)] at an average age of 152 days. Muscles differed in all fibre parameters, except the proportion and relative area of type IIx fibres. The LL muscle exhibited greater fibre cross-sectional areas of all fibre types, higher proportions of type IIb/IIB, and lower proportions of I and IIa fibres than the PM muscle in both staining techniques. These two muscles also differed marginally in moisture, crude protein and intramuscular fat content. The PM muscle showed a low correlation between fibre types and chemical composition, but the LL muscle showed moderate correlations between fibre CSA and area composition for moisture and ash content. After IHC staining, an increase in LL eye muscle area and drip loss were correlated with lower proportions of type I fibres, while a greater proportion of type IIx fibres resulted in increased LL eye muscle area and moisture content. Furthermore, a higher CSA of all fibre types in the LL decreased redness (a*) and moisture content of the muscle. Results showed that IHC is more appropriate than ATPase staining for the assessment of relationships between muscle fibre parameters and meat quality traits in pigs.https://cjas.agriculturejournals.cz/artkey/cjs-202006-0001_comparison-of-x202f-two-muscle-fibre-staining-techniques-and-their-relation-to-x202f-pork-quality-traits.phpatpase activityhistologyimmunohistochemistrymuscle fibre typemyosin heavy chain isoform
spellingShingle Nicole Lebedová
Tersia Needham
Jaroslav Čítek
Monika Okrouhlá
Kateřina Zadinová
Kamila Pokorná
Roman Stupka
Comparison of two muscle fibre staining techniques and their relation to pork quality traits
Czech Journal of Animal Science
atpase activity
histology
immunohistochemistry
muscle fibre type
myosin heavy chain isoform
title Comparison of two muscle fibre staining techniques and their relation to pork quality traits
title_full Comparison of two muscle fibre staining techniques and their relation to pork quality traits
title_fullStr Comparison of two muscle fibre staining techniques and their relation to pork quality traits
title_full_unstemmed Comparison of two muscle fibre staining techniques and their relation to pork quality traits
title_short Comparison of two muscle fibre staining techniques and their relation to pork quality traits
title_sort comparison of two muscle fibre staining techniques and their relation to pork quality traits
topic atpase activity
histology
immunohistochemistry
muscle fibre type
myosin heavy chain isoform
url https://cjas.agriculturejournals.cz/artkey/cjs-202006-0001_comparison-of-x202f-two-muscle-fibre-staining-techniques-and-their-relation-to-x202f-pork-quality-traits.php
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AT tersianeedham comparisonoftwomusclefibrestainingtechniquesandtheirrelationtoporkqualitytraits
AT jaroslavcitek comparisonoftwomusclefibrestainingtechniquesandtheirrelationtoporkqualitytraits
AT monikaokrouhla comparisonoftwomusclefibrestainingtechniquesandtheirrelationtoporkqualitytraits
AT katerinazadinova comparisonoftwomusclefibrestainingtechniquesandtheirrelationtoporkqualitytraits
AT kamilapokorna comparisonoftwomusclefibrestainingtechniquesandtheirrelationtoporkqualitytraits
AT romanstupka comparisonoftwomusclefibrestainingtechniquesandtheirrelationtoporkqualitytraits