Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products
The mycelial bacterium Streptomyces is a workhorse for producing natural products, serving as a key source of drugs and other valuable chemicals. However, its complicated life cycle, silent biosynthetic gene clusters (BGCs), and poorly characterized metabolic mechanisms limit efficient production of...
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2022-06-01
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| Series: | Engineering Microbiology |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2667370322000133 |
| _version_ | 1797788413585260544 |
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| author | Zuwei Xu Lihao Ji Wenxiu Tang Liang Guo Cong Gao Xiulai Chen Jia Liu Guipeng Hu Liming Liu |
| author_facet | Zuwei Xu Lihao Ji Wenxiu Tang Liang Guo Cong Gao Xiulai Chen Jia Liu Guipeng Hu Liming Liu |
| author_sort | Zuwei Xu |
| collection | DOAJ |
| description | The mycelial bacterium Streptomyces is a workhorse for producing natural products, serving as a key source of drugs and other valuable chemicals. However, its complicated life cycle, silent biosynthetic gene clusters (BGCs), and poorly characterized metabolic mechanisms limit efficient production of natural products. Therefore, a metabolic engineering strategy, including traditional and emerging tools from different disciplines, was developed to further enhance natural product synthesis by Streptomyces. Here, current trends in systems metabolic engineering, including tools and strategies, are reviewed. Particularly, this review focuses on recent developments in the selection of methods for regulating the Streptomyces life cycle, strategies for the activation of silent gene clusters, and the exploration of regulatory mechanisms governing antibiotic production. Finally, future challenges and prospects are discussed. |
| first_indexed | 2024-03-13T01:36:13Z |
| format | Article |
| id | doaj.art-c1b4b34aa9ed4c87bab03bd9a0d5cec3 |
| institution | Directory Open Access Journal |
| issn | 2667-3703 |
| language | English |
| last_indexed | 2024-03-13T01:36:13Z |
| publishDate | 2022-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Engineering Microbiology |
| spelling | doaj.art-c1b4b34aa9ed4c87bab03bd9a0d5cec32023-07-04T05:11:22ZengElsevierEngineering Microbiology2667-37032022-06-0122100022Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural productsZuwei Xu0Lihao Ji1Wenxiu Tang2Liang Guo3Cong Gao4Xiulai Chen5Jia Liu6Guipeng Hu7Liming Liu8State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, ChinaState Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi 214122, China; Corresponding author.The mycelial bacterium Streptomyces is a workhorse for producing natural products, serving as a key source of drugs and other valuable chemicals. However, its complicated life cycle, silent biosynthetic gene clusters (BGCs), and poorly characterized metabolic mechanisms limit efficient production of natural products. Therefore, a metabolic engineering strategy, including traditional and emerging tools from different disciplines, was developed to further enhance natural product synthesis by Streptomyces. Here, current trends in systems metabolic engineering, including tools and strategies, are reviewed. Particularly, this review focuses on recent developments in the selection of methods for regulating the Streptomyces life cycle, strategies for the activation of silent gene clusters, and the exploration of regulatory mechanisms governing antibiotic production. Finally, future challenges and prospects are discussed.http://www.sciencedirect.com/science/article/pii/S2667370322000133StreptomycesNatural productsMetabolic engineeringLife cycleBiosynthetic gene clusters |
| spellingShingle | Zuwei Xu Lihao Ji Wenxiu Tang Liang Guo Cong Gao Xiulai Chen Jia Liu Guipeng Hu Liming Liu Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products Engineering Microbiology Streptomyces Natural products Metabolic engineering Life cycle Biosynthetic gene clusters |
| title | Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products |
| title_full | Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products |
| title_fullStr | Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products |
| title_full_unstemmed | Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products |
| title_short | Metabolic engineering of Streptomyces to enhance the synthesis of valuable natural products |
| title_sort | metabolic engineering of streptomyces to enhance the synthesis of valuable natural products |
| topic | Streptomyces Natural products Metabolic engineering Life cycle Biosynthetic gene clusters |
| url | http://www.sciencedirect.com/science/article/pii/S2667370322000133 |
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