The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline
The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the m...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
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eLife Sciences Publications Ltd
2017-10-01
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| Series: | eLife |
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| Online Access: | https://elifesciences.org/articles/26116 |
| _version_ | 1811180692153827328 |
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| author | Alexis S Bailey Pedro J Batista Rebecca S Gold Y Grace Chen Dirk G de Rooij Howard Y Chang Margaret T Fuller |
| author_facet | Alexis S Bailey Pedro J Batista Rebecca S Gold Y Grace Chen Dirk G de Rooij Howard Y Chang Margaret T Fuller |
| author_sort | Alexis S Bailey |
| collection | DOAJ |
| description | The switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the mammalian Bgcn homolog YTHDC2 facilitates a clean switch from mitosis to meiosis in mouse germ cells, revealing a conserved role for YTHDC2 in this critical cell fate transition. YTHDC2-deficient male germ cells enter meiosis but have a mixed identity, maintaining expression of Cyclin A2 and failing to properly express many meiotic markers. Instead of continuing through meiotic prophase, the cells attempt an abnormal mitotic-like division and die. YTHDC2 binds multiple transcripts including Ccna2 and other mitotic transcripts, binds specific piRNA precursors, and interacts with RNA granule components, suggesting that proper progression of germ cells through meiosis is licensed by YTHDC2 through post-transcriptional regulation. |
| first_indexed | 2024-04-11T09:07:34Z |
| format | Article |
| id | doaj.art-c1bb0fbf955f4358a4ae2280819dbc35 |
| institution | Directory Open Access Journal |
| issn | 2050-084X |
| language | English |
| last_indexed | 2024-04-11T09:07:34Z |
| publishDate | 2017-10-01 |
| publisher | eLife Sciences Publications Ltd |
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| series | eLife |
| spelling | doaj.art-c1bb0fbf955f4358a4ae2280819dbc352022-12-22T04:32:36ZengeLife Sciences Publications LtdeLife2050-084X2017-10-01610.7554/eLife.26116The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germlineAlexis S Bailey0https://orcid.org/0000-0001-8838-8625Pedro J Batista1https://orcid.org/0000-0003-1055-2354Rebecca S Gold2https://orcid.org/0000-0002-2408-7716Y Grace Chen3https://orcid.org/0000-0003-3574-5734Dirk G de Rooij4https://orcid.org/0000-0003-3932-4419Howard Y Chang5https://orcid.org/0000-0002-9459-4393Margaret T Fuller6https://orcid.org/0000-0002-3804-4987Department of Developmental Biology, Stanford University School of Medicine, Stanford, United StatesCenter for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, United StatesDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, United StatesCenter for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, United StatesCenter for Reproductive Medicine, Academic Medical Center, University of Amsterdam, Amsterdam, NetherlandsCenter for Personal Dynamic Regulomes, Stanford University School of Medicine, Stanford, United StatesDepartment of Developmental Biology, Stanford University School of Medicine, Stanford, United StatesThe switch from mitosis to meiosis is the key event marking onset of differentiation in the germline stem cell lineage. In Drosophila, the translational repressor Bgcn is required for spermatogonia to stop mitosis and transition to meiotic prophase and the spermatocyte state. Here we show that the mammalian Bgcn homolog YTHDC2 facilitates a clean switch from mitosis to meiosis in mouse germ cells, revealing a conserved role for YTHDC2 in this critical cell fate transition. YTHDC2-deficient male germ cells enter meiosis but have a mixed identity, maintaining expression of Cyclin A2 and failing to properly express many meiotic markers. Instead of continuing through meiotic prophase, the cells attempt an abnormal mitotic-like division and die. YTHDC2 binds multiple transcripts including Ccna2 and other mitotic transcripts, binds specific piRNA precursors, and interacts with RNA granule components, suggesting that proper progression of germ cells through meiosis is licensed by YTHDC2 through post-transcriptional regulation.https://elifesciences.org/articles/26116germline stem cell lineagemitosis to meiosis transitionpost-transcriptional regulationYTHDC2Bgcnspermatogenesis |
| spellingShingle | Alexis S Bailey Pedro J Batista Rebecca S Gold Y Grace Chen Dirk G de Rooij Howard Y Chang Margaret T Fuller The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline eLife germline stem cell lineage mitosis to meiosis transition post-transcriptional regulation YTHDC2 Bgcn spermatogenesis |
| title | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
| title_full | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
| title_fullStr | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
| title_full_unstemmed | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
| title_short | The conserved RNA helicase YTHDC2 regulates the transition from proliferation to differentiation in the germline |
| title_sort | conserved rna helicase ythdc2 regulates the transition from proliferation to differentiation in the germline |
| topic | germline stem cell lineage mitosis to meiosis transition post-transcriptional regulation YTHDC2 Bgcn spermatogenesis |
| url | https://elifesciences.org/articles/26116 |
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