Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with Glycerol
Lotus <i>Receptaculum Nelumbinis</i> has been sparking wide research interests due to its rich phenolic compounds. In the present work, ultrasonic-assisted extraction coupled with glycerol was employed to extract phenolic compounds from <i>Receptaculum Nelumbinis</i> and the...
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MDPI AG
2021-01-01
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| Series: | Foods |
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| Online Access: | https://www.mdpi.com/2304-8158/10/2/239 |
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| author | Nina Bao Di Wang Xizhe Fu Hujun Xie Guizhen Gao Zisheng Luo |
| author_facet | Nina Bao Di Wang Xizhe Fu Hujun Xie Guizhen Gao Zisheng Luo |
| author_sort | Nina Bao |
| collection | DOAJ |
| description | Lotus <i>Receptaculum Nelumbinis</i> has been sparking wide research interests due to its rich phenolic compounds. In the present work, ultrasonic-assisted extraction coupled with glycerol was employed to extract phenolic compounds from <i>Receptaculum Nelumbinis</i> and the process was optimized using a response surface methodology with Box-Behnken design (BBD). The optimal conditions for the total phenolic content (TPC) extract were obtained: glycerol concentration of 40%, an extraction temperature of 66 °C, ultrasonic time of 44 min, and the solvent-to-solid ratio of 55 mL/g. Under these optimum extraction conditions, the extraction yield of TPC was 92.84 ± 2.13 mg gallic acid equivalents (GAE) /g. Besides, the antioxidant activities demonstrated the ability of free radical scavenging by four different methods that included 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing activity (RA) were 459.73 ± 7.07, 529.97 ± 7.30, 907.61 ± 20.28, and 983.66 ± 11.80 μmol TE/g, respectively. Six phenolic compounds were identified by ultra-high pressure liquid chromatography combined with triple-time-of-flight mass spectrophotometry (UPLC-Triple-TOF/MS) from the extracts. Meanwhile, Fourier transform infrared (FTIR) was conducted to identify the characteristic functional groups of the extracts and thus reflected the presence of polyphenols and flavonoids. Scanning electron microscopy (SEM) illustrated the microstructure difference of four treatments, which might explain the relationships between antioxidant activities and the structures of phenolic compounds. |
| first_indexed | 2024-03-09T03:47:45Z |
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| language | English |
| last_indexed | 2024-03-09T03:47:45Z |
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| series | Foods |
| spelling | doaj.art-c1bc99b26b2640e2b78378647d9edab52023-12-03T14:32:36ZengMDPI AGFoods2304-81582021-01-0110223910.3390/foods10020239Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with GlycerolNina Bao0Di Wang1Xizhe Fu2Hujun Xie3Guizhen Gao4Zisheng Luo5Suzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, Suzhou 234000, Anhui, ChinaZhejiang Key Laboratory for Agro-Food Processing, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, ChinaZhejiang Key Laboratory for Agro-Food Processing, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, ChinaSchool of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310058, Zhejiang, ChinaSuzhou Engineering and Technological Research Center of Natural Medicine and Functional Food, School of Biological and Food Engineering, Suzhou University, Suzhou 234000, Anhui, ChinaZhejiang Key Laboratory for Agro-Food Processing, National-Local Joint Engineering Laboratory of Intelligent Food Technology and Equipment, College of Biosystems Engineering and Food Science, Zhejiang University, Hangzhou 310058, Zhejiang, ChinaLotus <i>Receptaculum Nelumbinis</i> has been sparking wide research interests due to its rich phenolic compounds. In the present work, ultrasonic-assisted extraction coupled with glycerol was employed to extract phenolic compounds from <i>Receptaculum Nelumbinis</i> and the process was optimized using a response surface methodology with Box-Behnken design (BBD). The optimal conditions for the total phenolic content (TPC) extract were obtained: glycerol concentration of 40%, an extraction temperature of 66 °C, ultrasonic time of 44 min, and the solvent-to-solid ratio of 55 mL/g. Under these optimum extraction conditions, the extraction yield of TPC was 92.84 ± 2.13 mg gallic acid equivalents (GAE) /g. Besides, the antioxidant activities demonstrated the ability of free radical scavenging by four different methods that included 2,2-Diphenyl-1-picrylhydrazyl (DPPH), ferric reducing antioxidant power (FRAP), 2,2′-azinobis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS), and reducing activity (RA) were 459.73 ± 7.07, 529.97 ± 7.30, 907.61 ± 20.28, and 983.66 ± 11.80 μmol TE/g, respectively. Six phenolic compounds were identified by ultra-high pressure liquid chromatography combined with triple-time-of-flight mass spectrophotometry (UPLC-Triple-TOF/MS) from the extracts. Meanwhile, Fourier transform infrared (FTIR) was conducted to identify the characteristic functional groups of the extracts and thus reflected the presence of polyphenols and flavonoids. Scanning electron microscopy (SEM) illustrated the microstructure difference of four treatments, which might explain the relationships between antioxidant activities and the structures of phenolic compounds.https://www.mdpi.com/2304-8158/10/2/239<i>Receptaculum Nelumbinis</i>phenolic compoundultrasonic-assisted extractionantioxidant activityFourier transform infrared (FTIR)Scanning electron microscopy (SEM) |
| spellingShingle | Nina Bao Di Wang Xizhe Fu Hujun Xie Guizhen Gao Zisheng Luo Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with Glycerol Foods <i>Receptaculum Nelumbinis</i> phenolic compound ultrasonic-assisted extraction antioxidant activity Fourier transform infrared (FTIR) Scanning electron microscopy (SEM) |
| title | Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with Glycerol |
| title_full | Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with Glycerol |
| title_fullStr | Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with Glycerol |
| title_full_unstemmed | Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with Glycerol |
| title_short | Green Extraction of Phenolic Compounds from Lotus Seedpod (<i>Receptaculum Nelumbinis</i>) Assisted by Ultrasound Coupled with Glycerol |
| title_sort | green extraction of phenolic compounds from lotus seedpod i receptaculum nelumbinis i assisted by ultrasound coupled with glycerol |
| topic | <i>Receptaculum Nelumbinis</i> phenolic compound ultrasonic-assisted extraction antioxidant activity Fourier transform infrared (FTIR) Scanning electron microscopy (SEM) |
| url | https://www.mdpi.com/2304-8158/10/2/239 |
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