MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway
Objective(s): MAGI2-AS3 is a cancer suppressor gene of multiple malignancies. Acute lymphoblastic leukemia (ALL) is an important type of leukemia that especially occurs in children. Our work evaluated the modulation of MAGI2-AS3 in ALL.Materials and Methods: qPCR and Western blotting were adopted fo...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2022-01-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_19301_2880897d75bd5e6d764067c82942d63b.pdf |
| _version_ | 1819061046431711232 |
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| author | Xiao-Guang Chen Bing-Hua Dou Jin-Dou An Song Feng Na Liu Guang-Yao Sheng |
| author_facet | Xiao-Guang Chen Bing-Hua Dou Jin-Dou An Song Feng Na Liu Guang-Yao Sheng |
| author_sort | Xiao-Guang Chen |
| collection | DOAJ |
| description | Objective(s): MAGI2-AS3 is a cancer suppressor gene of multiple malignancies. Acute lymphoblastic leukemia (ALL) is an important type of leukemia that especially occurs in children. Our work evaluated the modulation of MAGI2-AS3 in ALL.Materials and Methods: qPCR and Western blotting were adopted for detection of target molecular expression. Growth and apoptosis were determined by CCK8 assay and Annexin V/PI staining. Glycolysis was detected by commercial kits. The direct binding between miR-452-5p and MAGI2-AS3 or FOXN3 was assessed by luciferase reporter assay. Tumor growth was measured in nude mice in vivo.Results: MAGI2-AS3 was down-regulated in ALL. Enforced expression of MAGI2-AS3 inhibited growth and glycolysis while promoting apoptosis of ALL cells. Moreover, MAGI2-AS3 up-regulated FOXN3 via sponging miR-452-5p. FOXN3 depletion abrogated MAGI2-AS3-mediated anti-cancer action. More importantly, MAGI2-AS3 repressed ALL cell growth in nude mice through regulation of miR-452-5p/FOXN3. Conclusion: MAGI2-AS3 inhibits ALL development via modulating miR-452-5p/FOXN3. |
| first_indexed | 2024-12-21T14:36:39Z |
| format | Article |
| id | doaj.art-c1bcfca6379b4883b26ea01ce2ba18e9 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-21T14:36:39Z |
| publishDate | 2022-01-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-c1bcfca6379b4883b26ea01ce2ba18e92022-12-21T19:00:19ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742022-01-01251465210.22038/ijbms.2021.58963.1309519301MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathwayXiao-Guang Chen0Bing-Hua Dou1Jin-Dou An2Song Feng3Na Liu4Guang-Yao Sheng5Department of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. ChinaDepartment of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. ChinaDepartment of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. ChinaDepartment of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. ChinaDepartment of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. ChinaDepartment of Pediatrics, The First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, Henan Province, P.R. ChinaObjective(s): MAGI2-AS3 is a cancer suppressor gene of multiple malignancies. Acute lymphoblastic leukemia (ALL) is an important type of leukemia that especially occurs in children. Our work evaluated the modulation of MAGI2-AS3 in ALL.Materials and Methods: qPCR and Western blotting were adopted for detection of target molecular expression. Growth and apoptosis were determined by CCK8 assay and Annexin V/PI staining. Glycolysis was detected by commercial kits. The direct binding between miR-452-5p and MAGI2-AS3 or FOXN3 was assessed by luciferase reporter assay. Tumor growth was measured in nude mice in vivo.Results: MAGI2-AS3 was down-regulated in ALL. Enforced expression of MAGI2-AS3 inhibited growth and glycolysis while promoting apoptosis of ALL cells. Moreover, MAGI2-AS3 up-regulated FOXN3 via sponging miR-452-5p. FOXN3 depletion abrogated MAGI2-AS3-mediated anti-cancer action. More importantly, MAGI2-AS3 repressed ALL cell growth in nude mice through regulation of miR-452-5p/FOXN3. Conclusion: MAGI2-AS3 inhibits ALL development via modulating miR-452-5p/FOXN3.https://ijbms.mums.ac.ir/article_19301_2880897d75bd5e6d764067c82942d63b.pdfallapoptosisfoxn3glycolysisgrowthmagi2-as3mir-452-5p |
| spellingShingle | Xiao-Guang Chen Bing-Hua Dou Jin-Dou An Song Feng Na Liu Guang-Yao Sheng MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway Iranian Journal of Basic Medical Sciences all apoptosis foxn3 glycolysis growth magi2-as3 mir-452-5p |
| title | MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway |
| title_full | MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway |
| title_fullStr | MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway |
| title_full_unstemmed | MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway |
| title_short | MAGI2-AS3 restrains proliferation, glycolysis, and triggers apoptosis in acute lymphoblastic leukemia via regulating miR-452-5p/FOXN3 pathway |
| title_sort | magi2 as3 restrains proliferation glycolysis and triggers apoptosis in acute lymphoblastic leukemia via regulating mir 452 5p foxn3 pathway |
| topic | all apoptosis foxn3 glycolysis growth magi2-as3 mir-452-5p |
| url | https://ijbms.mums.ac.ir/article_19301_2880897d75bd5e6d764067c82942d63b.pdf |
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