A Universal Strategy for the Efficient Expression of Nanobodies in <i>Pichia pastoris</i>

A Universal Strategy for the Efficient Expression of Nanobodies in <i>Pichia pastoris</i>

In recent years, nanobodies have played an increasingly crucial role in virus neutralization, ELISA detection, and medical imaging. This study aimed to explore a universal expression strategy in <i>Pichia pastoris</i> using three nanobodies, denoted Va, Vb, and Vc, as model proteins. Ini...

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Bibliographic Details
Main Authors: Yiheng Zheng, Bingkun Li, Shida Zhao, Jiawei Liu, Ding Li
Format: Article
Language:English
Published: MDPI AG 2024-01-01
Series:Fermentation
Subjects:
model nanobodies
antibiotic-free parental plasmids
expression components
gene dosage
molecular chaperones
H55 host
Online Access:https://www.mdpi.com/2311-5637/10/1/37

Internet

https://www.mdpi.com/2311-5637/10/1/37

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