Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end

Detection of dioxin-induced demethylation of mouse Cyp1a1 gene promoter by a new labeling method for short DNA fragments possessing 5'-methylcytosine at the end

Abstract Environmental factors stimulate alteration of DNA methylation level. Investigation of the genome-wide DNA methylation status is important for environmental health studies. We here designed a genomic DNA amplification and labeling protocol using a methylation-sensitive restriction enzyme Hin...

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Bibliographic Details
Main Authors: Hisaka Kurita, Toshiki Aiba, Toshiyuki Saito, Seiichiroh Ohsako
Format: Article
Language:English
Published: BMC 2018-01-01
Series:Genes and Environment
Subjects:
Restriction enzyme
Labeling
5′-Methylcytosine
Methylome
Online Access:http://link.springer.com/article/10.1186/s41021-017-0089-5

Internet

http://link.springer.com/article/10.1186/s41021-017-0089-5

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