An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis

Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin. The cell culture conditions have been optimized for inducing expression of keratinocyte differentiation markers. Cells are cultured in the...

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Bibliographic Details
Main Authors: Ratklao Siriwach, Anh Quynh Ngo, Shuh Narumiya, Dean Thumkeo
Format: Article
Language:English
Published: Elsevier 2022-12-01
Series:STAR Protocols
Subjects:
Sequence analysis
Cell Biology
Single Cell
Developmental biology
RNAseq
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166722007869

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http://www.sciencedirect.com/science/article/pii/S2666166722007869

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