An optimized protocol to identify keratinocyte subpopulations in vitro by single-cell RNA sequencing analysis
Summary: Here, we describe a protocol for single-cell isolation from the primary culture of normal human epidermal keratinocytes derived from neonatal foreskin. The cell culture conditions have been optimized for inducing expression of keratinocyte differentiation markers. Cells are cultured in the...
Main Authors: | Ratklao Siriwach, Anh Quynh Ngo, Shuh Narumiya, Dean Thumkeo |
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Format: | Article |
Language: | English |
Published: |
Elsevier
2022-12-01
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Series: | STAR Protocols |
Subjects: | |
Online Access: | http://www.sciencedirect.com/science/article/pii/S2666166722007869 |
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