Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus
The main objective of the study is to enhance the mevastatin production using Plackett–Burman (PB) and central composite design (CCD) by Aspergillus terreus in submerged fermentation (SmF). Eight nutrients were chosen for a PB design with 12 experimental runs. A maximum mevastatin production of 170....
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| Format: | Article |
| Language: | English |
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Elsevier
2015-06-01
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| Series: | Biotechnology Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2215017X15000211 |
| _version_ | 1811241669419335680 |
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| author | Mahin Basha Syed M. Rajasimman |
| author_facet | Mahin Basha Syed M. Rajasimman |
| author_sort | Mahin Basha Syed |
| collection | DOAJ |
| description | The main objective of the study is to enhance the mevastatin production using Plackett–Burman (PB) and central composite design (CCD) by Aspergillus terreus in submerged fermentation (SmF). Eight nutrients were chosen for a PB design with 12 experimental runs. A maximum mevastatin production of 170.4 mg L−1 was obtained in PB design. Response surface methodology (RSM) is a sequential procedure with an initial objective to lead the experimenter rapidly and efficiently along a path of improvement toward the general vicinity of the optimum. The individual and interactive effects of these variables were studied by conducting the fermentation run at randomly selected and different levels of all five factors. Experiments were conducted to optimize the medium constituents like glycerol, CuCl2·2H2O, FeSO4·7H2O, KH2PO4 and MgSO4·7H2O. At the optimum condition, a maximum mevastatin production of 701 mg L−1 was obtained. |
| first_indexed | 2024-04-12T13:39:30Z |
| format | Article |
| id | doaj.art-c208a82bc6a24a88813e2062b4ddcdeb |
| institution | Directory Open Access Journal |
| issn | 2215-017X |
| language | English |
| last_indexed | 2024-04-12T13:39:30Z |
| publishDate | 2015-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Biotechnology Reports |
| spelling | doaj.art-c208a82bc6a24a88813e2062b4ddcdeb2022-12-22T03:30:54ZengElsevierBiotechnology Reports2215-017X2015-06-016C12412810.1016/j.btre.2015.04.002Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreusMahin Basha Syed0M. Rajasimman1Environmental Engineering Lab, Nawab Shah Alam Khan College of Engineering and Technology, Malakpet, Hyderabad-500024, IndiaAnnamalai University, Annamalainagar-608002, Tamilnadu, IndiaThe main objective of the study is to enhance the mevastatin production using Plackett–Burman (PB) and central composite design (CCD) by Aspergillus terreus in submerged fermentation (SmF). Eight nutrients were chosen for a PB design with 12 experimental runs. A maximum mevastatin production of 170.4 mg L−1 was obtained in PB design. Response surface methodology (RSM) is a sequential procedure with an initial objective to lead the experimenter rapidly and efficiently along a path of improvement toward the general vicinity of the optimum. The individual and interactive effects of these variables were studied by conducting the fermentation run at randomly selected and different levels of all five factors. Experiments were conducted to optimize the medium constituents like glycerol, CuCl2·2H2O, FeSO4·7H2O, KH2PO4 and MgSO4·7H2O. At the optimum condition, a maximum mevastatin production of 701 mg L−1 was obtained.http://www.sciencedirect.com/science/article/pii/S2215017X15000211MevastatinPB designResponse surface methodologyAspergillus terreusSubmerged fermentation |
| spellingShingle | Mahin Basha Syed M. Rajasimman Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus Biotechnology Reports Mevastatin PB design Response surface methodology Aspergillus terreus Submerged fermentation |
| title | Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus |
| title_full | Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus |
| title_fullStr | Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus |
| title_full_unstemmed | Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus |
| title_short | Fermentative production and optimization of mevastatin in submerged fermentation using Aspergillus terreus |
| title_sort | fermentative production and optimization of mevastatin in submerged fermentation using aspergillus terreus |
| topic | Mevastatin PB design Response surface methodology Aspergillus terreus Submerged fermentation |
| url | http://www.sciencedirect.com/science/article/pii/S2215017X15000211 |
| work_keys_str_mv | AT mahinbashasyed fermentativeproductionandoptimizationofmevastatininsubmergedfermentationusingaspergillusterreus AT mrajasimman fermentativeproductionandoptimizationofmevastatininsubmergedfermentationusingaspergillusterreus |