In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography

In situ synthesis and unidirectional insertion of membrane proteins in liposome-immobilized silica stationary phase for rapid preparation of microaffinity chromatography

Cell membrane affinity chromatography has been widely applied in membrane protein (MP)-targeted drug screening and interaction analysis. However, in current methods, the MP sources are derived from cell lines or recombinant protein expression, which are time-consuming for cell culture or purificatio...

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Bibliographic Details
Main Authors: Yanqiu Gu, Rong Wang, Panpan Chen, Shengnan Li, Xinyi Chai, Chun Chen, Yue Liu, Yan Cao, Diya Lv, Zhanying Hong, Zhenyu Zhu, Yifeng Chai, Yongfang Yuan, Xiaofei Chen
Format: Article
Language:English
Published: Elsevier 2022-09-01
Series:Acta Pharmaceutica Sinica B
Subjects:
Affinity chromatography
In situ synthesis of membrane proteins
Unidirectional insertion
PDGFRβ
Drug screening
Ligand–protein interaction
Online Access:http://www.sciencedirect.com/science/article/pii/S2211383522001812

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http://www.sciencedirect.com/science/article/pii/S2211383522001812

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