Comparison between enzyme‐linked immunospot assay and intracellular cytokine flow cytometry assays for the evaluation of T cell response to SARS‐CoV‐2 after symptomatic COVID‐19

Comparison between enzyme‐linked immunospot assay and intracellular cytokine flow cytometry assays for the evaluation of T cell response to SARS‐CoV‐2 after symptomatic COVID‐19

Abstract Introduction Evaluation of different cell‐based assays for the study of adaptive immune responses against SARS‐CoV‐2 is crucial for studying long‐term and vaccine‐induced immunity. Methods Enzyme‐linked immunospot assay (ELISpot) and intracellular cytokine staining (ICS) using peptide pools...

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Bibliographic Details
Main Authors: Juliette Villemonteix, Laure Cohen, Amélie Guihot, Valérie Guérin, Clémentine Moulin, Marion Caseris, Agnès Carol, Stéphane Bonacorsi, Guislaine Carcelain
Format: Article
Language:English
Published: Wiley 2022-10-01
Series:Immunity, Inflammation and Disease
Subjects:
cellular immunity
ELISpot assay
intracellular cytokine staining assay
memory T cells
paucisymptomatic COVID‐19
SARS‐CoV‐2
Online Access:https://doi.org/10.1002/iid3.617

Internet

https://doi.org/10.1002/iid3.617

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