Improved Split TEV GPCR β-arrestin-2 Recruitment Assays via Systematic Analysis of Signal Peptide and β-arrestin Binding Motif Variants

Improved Split TEV GPCR β-arrestin-2 Recruitment Assays via Systematic Analysis of Signal Peptide and β-arrestin Binding Motif Variants

G protein-coupled receptors (GPCRs) are major disease-relevant drug targets; robust monitoring of their activities upon drug treatment is key to drug discovery. The split TEV cell-based assay technique monitors the interaction of an activated GPCR with β-arrestin-2 through TEV protein fragment compl...

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Bibliographic Details
Main Authors: Yuxin Wu, Isabelle V. von Hauff, Niels Jensen, Moritz J. Rossner, Michael C. Wehr
Format: Article
Language:English
Published: MDPI AG 2022-12-01
Series:Biosensors
Subjects:
GPCR
drug screening
drug discovery
cell-based assay
split TEV technique
Online Access:https://www.mdpi.com/2079-6374/13/1/48

Internet

https://www.mdpi.com/2079-6374/13/1/48

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