The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions
Abstract Background It is increasingly recognized that conventional food production systems are not able to meet the globally increasing protein needs, resulting in overexploitation and depletion of resources, and environmental degradation. In this context, microbial biomass has emerged as a promisi...
| Main Authors: | , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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BMC
2023-12-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02265-1 |
| _version_ | 1827581313114177536 |
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| author | Myrsini Sakarika Frederiek-Maarten Kerckhof Lotte Van Peteghem Alexandra Pereira Tim Van Den Bossche Robbin Bouwmeester Ralf Gabriels Delphi Van Haver Barbara Ulčar Lennart Martens Francis Impens Nico Boon Ramon Ganigué Korneel Rabaey |
| author_facet | Myrsini Sakarika Frederiek-Maarten Kerckhof Lotte Van Peteghem Alexandra Pereira Tim Van Den Bossche Robbin Bouwmeester Ralf Gabriels Delphi Van Haver Barbara Ulčar Lennart Martens Francis Impens Nico Boon Ramon Ganigué Korneel Rabaey |
| author_sort | Myrsini Sakarika |
| collection | DOAJ |
| description | Abstract Background It is increasingly recognized that conventional food production systems are not able to meet the globally increasing protein needs, resulting in overexploitation and depletion of resources, and environmental degradation. In this context, microbial biomass has emerged as a promising sustainable protein alternative. Nevertheless, often no consideration is given on the fact that the cultivation conditions affect the composition of microbial cells, and hence their quality and nutritional value. Apart from the properties and nutritional quality of the produced microbial food (ingredient), this can also impact its sustainability. To qualitatively assess these aspects, here, we investigated the link between substrate availability, growth rate, cell composition and size of Cupriavidus necator and Komagataella phaffii. Results Biomass with decreased nucleic acid and increased protein content was produced at low growth rates. Conversely, high rates resulted in larger cells, which could enable more efficient biomass harvesting. The proteome allocation varied across the different growth rates, with more ribosomal proteins at higher rates, which could potentially affect the techno-functional properties of the biomass. Considering the distinct amino acid profiles established for the different cellular components, variations in their abundance impacts the product quality leading to higher cysteine and phenylalanine content at low growth rates. Therefore, we hint that costly external amino acid supplementations that are often required to meet the nutritional needs could be avoided by carefully applying conditions that enable targeted growth rates. Conclusion In summary, we demonstrate tradeoffs between nutritional quality and production rate, and we discuss the microbial biomass properties that vary according to the growth conditions. |
| first_indexed | 2024-03-08T22:34:37Z |
| format | Article |
| id | doaj.art-c21a30b896344243ae9bf3489916e70f |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-03-08T22:34:37Z |
| publishDate | 2023-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-c21a30b896344243ae9bf3489916e70f2023-12-17T12:34:10ZengBMCMicrobial Cell Factories1475-28592023-12-0122111510.1186/s12934-023-02265-1The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditionsMyrsini Sakarika0Frederiek-Maarten Kerckhof1Lotte Van Peteghem2Alexandra Pereira3Tim Van Den Bossche4Robbin Bouwmeester5Ralf Gabriels6Delphi Van Haver7Barbara Ulčar8Lennart Martens9Francis Impens10Nico Boon11Ramon Ganigué12Korneel Rabaey13Center for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityCenter for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityCenter for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityCenter for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityVIB-UGent Center for Medical Biotechnology, VIBVIB-UGent Center for Medical Biotechnology, VIBVIB-UGent Center for Medical Biotechnology, VIBVIB-UGent Center for Medical Biotechnology, VIBCenter for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityVIB-UGent Center for Medical Biotechnology, VIBVIB-UGent Center for Medical Biotechnology, VIBCenter for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityCenter for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityCenter for Microbial Ecology and Technology (CMET), Faculty of Bioscience Engineering, Ghent UniversityAbstract Background It is increasingly recognized that conventional food production systems are not able to meet the globally increasing protein needs, resulting in overexploitation and depletion of resources, and environmental degradation. In this context, microbial biomass has emerged as a promising sustainable protein alternative. Nevertheless, often no consideration is given on the fact that the cultivation conditions affect the composition of microbial cells, and hence their quality and nutritional value. Apart from the properties and nutritional quality of the produced microbial food (ingredient), this can also impact its sustainability. To qualitatively assess these aspects, here, we investigated the link between substrate availability, growth rate, cell composition and size of Cupriavidus necator and Komagataella phaffii. Results Biomass with decreased nucleic acid and increased protein content was produced at low growth rates. Conversely, high rates resulted in larger cells, which could enable more efficient biomass harvesting. The proteome allocation varied across the different growth rates, with more ribosomal proteins at higher rates, which could potentially affect the techno-functional properties of the biomass. Considering the distinct amino acid profiles established for the different cellular components, variations in their abundance impacts the product quality leading to higher cysteine and phenylalanine content at low growth rates. Therefore, we hint that costly external amino acid supplementations that are often required to meet the nutritional needs could be avoided by carefully applying conditions that enable targeted growth rates. Conclusion In summary, we demonstrate tradeoffs between nutritional quality and production rate, and we discuss the microbial biomass properties that vary according to the growth conditions.https://doi.org/10.1186/s12934-023-02265-1Amino acid profileGrowth rateNucleic acidNutritional qualityProtein profile |
| spellingShingle | Myrsini Sakarika Frederiek-Maarten Kerckhof Lotte Van Peteghem Alexandra Pereira Tim Van Den Bossche Robbin Bouwmeester Ralf Gabriels Delphi Van Haver Barbara Ulčar Lennart Martens Francis Impens Nico Boon Ramon Ganigué Korneel Rabaey The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions Microbial Cell Factories Amino acid profile Growth rate Nucleic acid Nutritional quality Protein profile |
| title | The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions |
| title_full | The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions |
| title_fullStr | The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions |
| title_full_unstemmed | The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions |
| title_short | The nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions |
| title_sort | nutritional composition and cell size of microbial biomass for food applications are defined by the growth conditions |
| topic | Amino acid profile Growth rate Nucleic acid Nutritional quality Protein profile |
| url | https://doi.org/10.1186/s12934-023-02265-1 |
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