Targeted DNA methylation in vivo using an engineered dCas9-MQ1 fusion protein
Understanding how DNA methylation regulates gene expression requires the capacity to deploy it to regions of interest. The authors generate a highly rapid and locus-specific CpG methylation tool by fusing dCas9 to MQ1 DNA methyltransferase and show efficacy at multiple sitesin vitro and in vivo.
| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2017-07-01
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| Series: | Nature Communications |
| Online Access: | https://doi.org/10.1038/ncomms16026 |