Direct quantification of cell-free, circulating DNA from unpurified plasma.

Cell-free DNA (cfDNA) in body tissues or fluids is extensively investigated in clinical medicine and other research fields. In this article we provide a direct quantitative real-time PCR (qPCR) as a sensitive tool for the measurement of cfDNA from plasma without previous DNA extraction, which is kno...

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Bibliographic Details
Main Authors: Sarah Breitbach, Suzan Tug, Susanne Helmig, Daniela Zahn, Thomas Kubiak, Matthias Michal, Tommaso Gori, Tobias Ehlert, Thomas Beiter, Perikles Simon
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0087838&type=printable