Protocol for ex vivo culture of patient-derived tumor fragments

Protocol for ex vivo culture of patient-derived tumor fragments

Summary: The lack of suitable models currently hampers our understanding of how the tumor microenvironment responds to immunotherapy treatment. Here, we present a protocol for ex vivo culture of patient-derived tumor fragments (PDTFs). We describe the steps for tumor collection, generation and cryop...

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Bibliographic Details
Main Authors: Lisanne M. Roelofsen, Paula Voabil, Marjolein de Bruijn, Petra Herzig, Alfred Zippelius, Ton N. Schumacher, Daniela S. Thommen
Format: Article
Language:English
Published: Elsevier 2023-06-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell Culture
Single Cell
Cancer
Immunology
Biotechnology and Bioengineering
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166723002496

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http://www.sciencedirect.com/science/article/pii/S2666166723002496

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