3D visualization of macromolecule synthesis

3D visualization of macromolecule synthesis

Measuring nascent macromolecular synthesis in vivo is key to understanding how cells and tissues progress through development and respond to external cues. Here we perform in vivo injection of alkyne- or azide-modified analogs of thymidine, uridine, methionine, and glucosamine to label nascent synth...

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Bibliographic Details
Main Authors: Timothy J Duerr, Ester Comellas, Eun Kyung Jeon, Johanna E Farkas, Marylou Joetzjer, Julien Garnier, Sandra J Shefelbine, James R Monaghan
Format: Article
Language:English
Published: eLife Sciences Publications Ltd 2020-10-01
Series:eLife
Subjects:
Axolotl
light sheet fluorescence microscopy
click-chemistry
macromolecules
whole mount
Online Access:https://elifesciences.org/articles/60354

Internet

https://elifesciences.org/articles/60354

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