Stubborn contaminants: influence of detergents on the purity of the multidrug ABC transporter BmrA.

Despite the growing interest in membrane proteins, their crystallization remains a major challenge. In the course of a crystallographic study on the multidrug ATP-binding cassette transporter BmrA, mass spectral analyses on samples purified with six selected detergents revealed unexpected protein co...

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Bibliographic Details
Main Authors: Benjamin Wiseman, Arnaud Kilburg, Vincent Chaptal, Gina Catalina Reyes-Mejia, Jonathan Sarwan, Pierre Falson, Jean-Michel Jault
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC4269414?pdf=render