A Simple, Semi-Quantitative Acyl Biotin Exchange-Based Method to Detect Protein S-Palmitoylation Levels

A Simple, Semi-Quantitative Acyl Biotin Exchange-Based Method to Detect Protein S-Palmitoylation Levels

Protein S-palmitoylation is a reversible post-translational lipidation in which palmitic acid (16:0) is added to protein cysteine residue by a covalent thioester bond. This modification plays an active role in membrane targeting of soluble proteins, protein–protein interaction, protein trafficking,...

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Bibliographic Details
Main Authors: Valentina Buffa, Giorgia Adamo, Sabrina Picciotto, Antonella Bongiovanni, Daniele P. Romancino
Format: Article
Language:English
Published: MDPI AG 2023-03-01
Series:Membranes
Subjects:
membrane protein
post-translational lipidation
S-palmitoylation
acyl biotin exchange (ABE)
Online Access:https://www.mdpi.com/2077-0375/13/3/361

Internet

https://www.mdpi.com/2077-0375/13/3/361

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