Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean Seed

Soybean (<i>Glycine max</i> (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds...

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Main Authors: Yuchen Zhang, Qingyu Wang, Yajing Liu, Shuo Dong, Yongqiang Zhang, Youcheng Zhu, Yu Tian, Jingwen Li, Zhuoyi Wang, Ying Wang, Fan Yan
Format: Article
Language:English
Published: MDPI AG 2023-09-01
Series:International Journal of Molecular Sciences
Subjects:
Online Access:https://www.mdpi.com/1422-0067/24/18/14125
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author Yuchen Zhang
Qingyu Wang
Yajing Liu
Shuo Dong
Yongqiang Zhang
Youcheng Zhu
Yu Tian
Jingwen Li
Zhuoyi Wang
Ying Wang
Fan Yan
author_facet Yuchen Zhang
Qingyu Wang
Yajing Liu
Shuo Dong
Yongqiang Zhang
Youcheng Zhu
Yu Tian
Jingwen Li
Zhuoyi Wang
Ying Wang
Fan Yan
author_sort Yuchen Zhang
collection DOAJ
description Soybean (<i>Glycine max</i> (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine γ-synthase 2 (<i>GmCGS2</i>), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar “Jack”, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that <i>GmCGS2</i> plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds.
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spelling doaj.art-c254df1c9b854925812f2b988075dc9a2023-11-19T11:08:16ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-09-0124181412510.3390/ijms241814125Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean SeedYuchen Zhang0Qingyu Wang1Yajing Liu2Shuo Dong3Yongqiang Zhang4Youcheng Zhu5Yu Tian6Jingwen Li7Zhuoyi Wang8Ying Wang9Fan Yan10College of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaCollege of Plant Science, Jilin University, Changchun 130012, ChinaSoybean (<i>Glycine max</i> (L.) Merr.) is an important source of plant protein, the nutritional quality of which is considerably affected by the content of the sulfur-containing amino acid, methionine (Met). To improve the quality of soybean protein and increase the Met content in seeds, soybean cystathionine γ-synthase 2 (<i>GmCGS2</i>), the first unique enzyme in Met biosynthesis, was overexpressed in the soybean cultivar “Jack”, producing three transgenic lines (OE3, OE4, and OE10). We detected a considerable increase in the content of free Met and other free amino acids in the developing seeds of the three transgenic lines at the 15th and 75th days after flowering (15D and 75D). In addition, transcriptome analysis showed that the expression of genes related to Met biosynthesis from the aspartate-family pathway and S-methyl Met cycle was promoted in developing green seeds of OE10. Ultimately, the accumulation of total amino acids and soluble proteins in transgenic mature seeds was promoted. Altogether, these results indicated that <i>GmCGS2</i> plays an important role in Met biosynthesis, by providing a basis for improving the nutritional quality of soybean seeds.https://www.mdpi.com/1422-0067/24/18/14125cystathionine γ-synthase<i>Glycine max</i>methionine biosynthesissoybean proteinsulfur-containing amino acidmethionine
spellingShingle Yuchen Zhang
Qingyu Wang
Yajing Liu
Shuo Dong
Yongqiang Zhang
Youcheng Zhu
Yu Tian
Jingwen Li
Zhuoyi Wang
Ying Wang
Fan Yan
Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean Seed
International Journal of Molecular Sciences
cystathionine γ-synthase
<i>Glycine max</i>
methionine biosynthesis
soybean protein
sulfur-containing amino acid
methionine
title Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean Seed
title_full Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean Seed
title_fullStr Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean Seed
title_full_unstemmed Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean Seed
title_short Overexpressing <i>GmCGS2</i> Improves Total Amino Acid and Protein Content in Soybean Seed
title_sort overexpressing i gmcgs2 i improves total amino acid and protein content in soybean seed
topic cystathionine γ-synthase
<i>Glycine max</i>
methionine biosynthesis
soybean protein
sulfur-containing amino acid
methionine
url https://www.mdpi.com/1422-0067/24/18/14125
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