Cryopreservation of human cerebral microvascular endothelial cells and astrocytes in suspension and monolayers.

The blood-brain barrier (BBB) keeps pathogens and toxins out of the brain but also impedes the entry of pharmaceuticals. Human cerebral microvascular endothelial cells (hCMECs) and astrocytes are the main functional cell components of the BBB. Although available commercially as cryopreserved cells i...

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Bibliographic Details
Main Authors: Leah A Marquez-Curtis, Reid Bokenfohr, Locksley E McGann, Janet A W Elliott
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2021-01-01
Series:PLoS ONE
Online Access:https://doi.org/10.1371/journal.pone.0249814