Detection and Quantification of SARS-CoV-2 Receptor Binding Domain Neutralization by a Sensitive Competitive ELISA Assay

Detection and Quantification of SARS-CoV-2 Receptor Binding Domain Neutralization by a Sensitive Competitive ELISA Assay

This protocol describes an ELISA-based procedure for accurate measurement of SARS-CoV-2 spike protein-receptor binding domain (RBD) neutralization efficacy by murine immune serum. The procedure requires a small amount of S-protein/RBD and angiotensin converting enzyme-2 (ACE2). A high-throughput, si...

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Bibliographic Details
Main Authors: Ahmed O. Shalash, Armira Azuar, Harrison Y. R. Madge, Naphak Modhiran, Alberto A. Amarilla, Benjamin Liang, Alexander A. Khromykh, Daniel Watterson, Paul R. Young, Istvan Toth, Mariusz Skwarczynski
Format: Article
Language:English
Published: MDPI AG 2021-12-01
Series:Vaccines
Subjects:
SARS-CoV-2
receptor binding domain
spike protein
binding inhibition assay
neutralization assay
competitive ELISA
Online Access:https://www.mdpi.com/2076-393X/9/12/1493

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https://www.mdpi.com/2076-393X/9/12/1493

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