β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs.
Oocyte senescence alters the shape and function, thereby weakening the fertilization potential. Nicotinamide mononucleotide (NMN) reverses age-related dysfunctions in various organs. Studies had shown long-term administration of NMN reduced the physiological decline associated in aged mice and rever...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2023-01-01
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| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0291640&type=printable |
| _version_ | 1797635293617061888 |
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| author | Leyi Li Qinghe Han Yurong Chen Meng Zhang Luyao Wang Xinglan An Sheng Zhang Yanhui Zhai Xiangpeng Dai Bo Tang Ziyi Li Guanghong Xie |
| author_facet | Leyi Li Qinghe Han Yurong Chen Meng Zhang Luyao Wang Xinglan An Sheng Zhang Yanhui Zhai Xiangpeng Dai Bo Tang Ziyi Li Guanghong Xie |
| author_sort | Leyi Li |
| collection | DOAJ |
| description | Oocyte senescence alters the shape and function, thereby weakening the fertilization potential. Nicotinamide mononucleotide (NMN) reverses age-related dysfunctions in various organs. Studies had shown long-term administration of NMN reduced the physiological decline associated in aged mice and reversed the aging of the ovaries. However, the protective effect of NMN on aged porcine oocytes is still unclear. In this study, we investigated the effects of NMN on aging porcine oocytes and subsequent embryonic development. We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro. NMN supplementation significantly reduced reactive oxygen species (ROS) levels in senescence oocytes and increased the mRNA levels of antioxidant genes SOD1 and Cat. The mitochondrial membrane potential of aged oocytes treated with NMN was increased compared with that of untreated oocytes. In addition, the mRNA level of apoptosis-related gene Bax was significantly decreased in senescence oocytes treated with NMN, while the mRNA level of anti-apoptosis-related gene BCL-2 was significantly increased. Furthermore, NMN supplementation enhanced the subsequent development ability of senescent oocytes during in vitro aging. Compared with untreated senescent oocytes, the blastocyst formation rate and pluripotent genes of senescent oocytes treated with NMN were significantly increased. Taken together, these results suggest that NMN is beneficial for delaying the aging process in porcine oocytes. |
| first_indexed | 2024-03-11T12:19:07Z |
| format | Article |
| id | doaj.art-c28f5bad14444374973bc61af6a756db |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-03-11T12:19:07Z |
| publishDate | 2023-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c28f5bad14444374973bc61af6a756db2023-11-07T05:34:27ZengPublic Library of Science (PLoS)PLoS ONE1932-62032023-01-011810e029164010.1371/journal.pone.0291640β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs.Leyi LiQinghe HanYurong ChenMeng ZhangLuyao WangXinglan AnSheng ZhangYanhui ZhaiXiangpeng DaiBo TangZiyi LiGuanghong XieOocyte senescence alters the shape and function, thereby weakening the fertilization potential. Nicotinamide mononucleotide (NMN) reverses age-related dysfunctions in various organs. Studies had shown long-term administration of NMN reduced the physiological decline associated in aged mice and reversed the aging of the ovaries. However, the protective effect of NMN on aged porcine oocytes is still unclear. In this study, we investigated the effects of NMN on aging porcine oocytes and subsequent embryonic development. We established a model of senescence of porcine oocytes after ovulation by extending the culture time in vitro. NMN supplementation significantly reduced reactive oxygen species (ROS) levels in senescence oocytes and increased the mRNA levels of antioxidant genes SOD1 and Cat. The mitochondrial membrane potential of aged oocytes treated with NMN was increased compared with that of untreated oocytes. In addition, the mRNA level of apoptosis-related gene Bax was significantly decreased in senescence oocytes treated with NMN, while the mRNA level of anti-apoptosis-related gene BCL-2 was significantly increased. Furthermore, NMN supplementation enhanced the subsequent development ability of senescent oocytes during in vitro aging. Compared with untreated senescent oocytes, the blastocyst formation rate and pluripotent genes of senescent oocytes treated with NMN were significantly increased. Taken together, these results suggest that NMN is beneficial for delaying the aging process in porcine oocytes.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0291640&type=printable |
| spellingShingle | Leyi Li Qinghe Han Yurong Chen Meng Zhang Luyao Wang Xinglan An Sheng Zhang Yanhui Zhai Xiangpeng Dai Bo Tang Ziyi Li Guanghong Xie β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs. PLoS ONE |
| title | β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs. |
| title_full | β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs. |
| title_fullStr | β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs. |
| title_full_unstemmed | β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs. |
| title_short | β-nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs. |
| title_sort | β nicotinamide mononucleotide rescues the quality of aged oocyte and improves subsequent embryo development in pigs |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0291640&type=printable |
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