Using the Autofluorescence Finder on the Sony ID7000TM Spectral Cell Analyzer to Identify and Unmix Multiple Highly Autofluorescent Murine Lung Populations

Using the Autofluorescence Finder on the Sony ID7000TM Spectral Cell Analyzer to Identify and Unmix Multiple Highly Autofluorescent Murine Lung Populations

Autofluorescence (AF) is a feature of all cell types, though some have more than others. In tissues with complex heterogeneous cellularity, AF is frequently a source of high background, masking faint fluorescent signals and reducing the available dynamic range of detectors for detecting fluorescence...

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Bibliographic Details
Main Authors: Nicholas Wanner, Jerry Barnhart, Nicholas Apostolakis, Violetta Zlojutro, Kewal Asosingh
Format: Article
Language:English
Published: Frontiers Media S.A. 2022-03-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
autofluorescence
spectral flow cytometry
murine lung
Sony ID7000
autofluorescence finder
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2022.827987/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2022.827987/full

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