Rapid Enrichment and Isolation of Polyphosphate-Accumulating Organisms Through 4’6-Diamidino-2-Phenylindole (DAPI) Staining With Fluorescence-Activated Cell Sorting (FACS)

Rapid Enrichment and Isolation of Polyphosphate-Accumulating Organisms Through 4’6-Diamidino-2-Phenylindole (DAPI) Staining With Fluorescence-Activated Cell Sorting (FACS)

Screening for bacteria with abilities to accumulate valuable intracellular compounds from an environmental community is difficult and requires strategic methods. Combining the experimental procedure for phenotyping living cells in a microbial community with the cell recovery necessary for further cu...

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Bibliographic Details
Main Authors: Mia Terashima, Yoichi Kamagata, Souichiro Kato
Format: Article
Language:English
Published: Frontiers Media S.A. 2020-04-01
Series:Frontiers in Microbiology
Subjects:
Polyphosphate-accumulating organisms
flow cytometry
wastewater sludge
bacteria isolation
DAPI
Online Access:https://www.frontiersin.org/article/10.3389/fmicb.2020.00793/full

Internet

https://www.frontiersin.org/article/10.3389/fmicb.2020.00793/full

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