Efficient inactivation of symbiotic nitrogen fixation related genes in Lotus japonicus using CRISPR-Cas9

Efficient inactivation of symbiotic nitrogen fixation related genes in Lotus japonicus using CRISPR-Cas9

The targeted genome editing technique, CRISPR/Cas9 system, has been widely used to modify genes of interest in a predictable and precise manner. In this study, we describe the CRISPR/Cas9-mediated efficient editing of representative SNF (symbiotic nitrogen fixation) related genes in the model legume...

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Bibliographic Details
Main Authors: Longxiang Wang, Longlong Wang, Qian Tan, Qiuling Fan, Hui Zhu, Zonglie Hong, Zhongming Zhang, Deqiang Duanmu
Format: Article
Language:English
Published: Frontiers Media S.A. 2016-08-01
Series:Frontiers in Plant Science
Subjects:
Leghemoglobin
Nitrogen Fixation
Symbiosis
Lotus japonicus
Genome editing
CRISPR/Cas9
Online Access:http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01333/full

Internet

http://journal.frontiersin.org/Journal/10.3389/fpls.2016.01333/full

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