Multiplexed Sequence Encoding: A Framework for DNA Communication.
Synthetic DNA has great propensity for efficiently and stably storing non-biological information. With DNA writing and reading technologies rapidly advancing, new applications for synthetic DNA are emerging in data storage and communication. Traditionally, DNA communication has focused on the encodi...
| Huvudupphovsmän: | , , |
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| Materialtyp: | Artikel |
| Språk: | English |
| Publicerad: |
Public Library of Science (PLoS)
2016-01-01
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| Serie: | PLoS ONE |
| Länkar: | http://europepmc.org/articles/PMC4822886?pdf=render |
| _version_ | 1829118190574108672 |
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| author | Bijan Zakeri Peter A Carr Timothy K Lu |
| author_facet | Bijan Zakeri Peter A Carr Timothy K Lu |
| author_sort | Bijan Zakeri |
| collection | DOAJ |
| description | Synthetic DNA has great propensity for efficiently and stably storing non-biological information. With DNA writing and reading technologies rapidly advancing, new applications for synthetic DNA are emerging in data storage and communication. Traditionally, DNA communication has focused on the encoding and transfer of complete sets of information. Here, we explore the use of DNA for the communication of short messages that are fragmented across multiple distinct DNA molecules. We identified three pivotal points in a communication-data encoding, data transfer & data extraction-and developed novel tools to enable communication via molecules of DNA. To address data encoding, we designed DNA-based individualized keyboards (iKeys) to convert plaintext into DNA, while reducing the occurrence of DNA homopolymers to improve synthesis and sequencing processes. To address data transfer, we implemented a secret-sharing system-Multiplexed Sequence Encoding (MuSE)-that conceals messages between multiple distinct DNA molecules, requiring a combination key to reveal messages. To address data extraction, we achieved the first instance of chromatogram patterning through multiplexed sequencing, thereby enabling a new method for data extraction. We envision these approaches will enable more widespread communication of information via DNA. |
| first_indexed | 2024-12-13T10:17:59Z |
| format | Article |
| id | doaj.art-c2e28e7b31f44cf0a0cce1df7d4add7f |
| institution | Directory Open Access Journal |
| issn | 1932-6203 |
| language | English |
| last_indexed | 2024-12-13T10:17:59Z |
| publishDate | 2016-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj.art-c2e28e7b31f44cf0a0cce1df7d4add7f2022-12-21T23:51:16ZengPublic Library of Science (PLoS)PLoS ONE1932-62032016-01-01114e015277410.1371/journal.pone.0152774Multiplexed Sequence Encoding: A Framework for DNA Communication.Bijan ZakeriPeter A CarrTimothy K LuSynthetic DNA has great propensity for efficiently and stably storing non-biological information. With DNA writing and reading technologies rapidly advancing, new applications for synthetic DNA are emerging in data storage and communication. Traditionally, DNA communication has focused on the encoding and transfer of complete sets of information. Here, we explore the use of DNA for the communication of short messages that are fragmented across multiple distinct DNA molecules. We identified three pivotal points in a communication-data encoding, data transfer & data extraction-and developed novel tools to enable communication via molecules of DNA. To address data encoding, we designed DNA-based individualized keyboards (iKeys) to convert plaintext into DNA, while reducing the occurrence of DNA homopolymers to improve synthesis and sequencing processes. To address data transfer, we implemented a secret-sharing system-Multiplexed Sequence Encoding (MuSE)-that conceals messages between multiple distinct DNA molecules, requiring a combination key to reveal messages. To address data extraction, we achieved the first instance of chromatogram patterning through multiplexed sequencing, thereby enabling a new method for data extraction. We envision these approaches will enable more widespread communication of information via DNA.http://europepmc.org/articles/PMC4822886?pdf=render |
| spellingShingle | Bijan Zakeri Peter A Carr Timothy K Lu Multiplexed Sequence Encoding: A Framework for DNA Communication. PLoS ONE |
| title | Multiplexed Sequence Encoding: A Framework for DNA Communication. |
| title_full | Multiplexed Sequence Encoding: A Framework for DNA Communication. |
| title_fullStr | Multiplexed Sequence Encoding: A Framework for DNA Communication. |
| title_full_unstemmed | Multiplexed Sequence Encoding: A Framework for DNA Communication. |
| title_short | Multiplexed Sequence Encoding: A Framework for DNA Communication. |
| title_sort | multiplexed sequence encoding a framework for dna communication |
| url | http://europepmc.org/articles/PMC4822886?pdf=render |
| work_keys_str_mv | AT bijanzakeri multiplexedsequenceencodingaframeworkfordnacommunication AT peteracarr multiplexedsequenceencodingaframeworkfordnacommunication AT timothyklu multiplexedsequenceencodingaframeworkfordnacommunication |