Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol
We used a mitochondria-penetrating nitroxide, mito-TEMPO, as a contrast probe for imaging of kidney dysfunction in mice, based on the redox-imbalance and oxidative stress in the renal tissues. Kidney dysfunction was triggered by hypercholesterolemia. The mice were divided in three groups: (i) on nor...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Biotechnology & Biotechnological Equipment |
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| Online Access: | http://dx.doi.org/10.1080/13102818.2019.1573153 |
| _version_ | 1818258114876538880 |
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| author | Dessislava Lazarova Sayaka Shibata Itsuko Ishii Genoveva Zlateva Zhivko Zhelev Ichio Aoki Rumiana Bakalova |
| author_facet | Dessislava Lazarova Sayaka Shibata Itsuko Ishii Genoveva Zlateva Zhivko Zhelev Ichio Aoki Rumiana Bakalova |
| author_sort | Dessislava Lazarova |
| collection | DOAJ |
| description | We used a mitochondria-penetrating nitroxide, mito-TEMPO, as a contrast probe for imaging of kidney dysfunction in mice, based on the redox-imbalance and oxidative stress in the renal tissues. Kidney dysfunction was triggered by hypercholesterolemia. The mice were divided in three groups: (i) on normal diet (ND; control); (ii) on cholesterol diet (CD); (iii) on cholesterol plus cholestyramine diet (CC). CD mice showed increased plasma levels of total cholesterol and non-HDL-cholesterol, as well as increased serum levels of blood urea nitrogen, uric acid and creatinine, compared to ND mice. CC mice showed slightly increased plasma levels of total cholesterol and HDL-cholesterol, but not non-HDL-cholesterol, compared to ND mice. The serum levels of blood urea nitrogen, uric acid and creatinine in CC mice were equal to those in ND mice. The MRI signal of mito-TEMPO in the kidneys was characterized by: high intensity and long life-time in CD mice, indicating a high oxidative capacity of renal tissues; poor intensity and short life-time in ND mice, indicating a high reducing capacity of renal tissues; moderate intensity and relatively short life-time in CC mice, which shows the protective effect of lipid-lowering agents against oxidative damage. The data suggest that hypercholesterolemia induces redox-imbalance and oxidative stress in kidneys and this process could be visualized using MRI and mito-TEMPO as a redox-sensitive contrast substance. |
| first_indexed | 2024-12-12T17:54:24Z |
| format | Article |
| id | doaj.art-c2ed0ada69e44e6a9a510dc947734f90 |
| institution | Directory Open Access Journal |
| issn | 1310-2818 1314-3530 |
| language | English |
| last_indexed | 2024-12-12T17:54:24Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Biotechnology & Biotechnological Equipment |
| spelling | doaj.art-c2ed0ada69e44e6a9a510dc947734f902022-12-22T00:16:44ZengTaylor & Francis GroupBiotechnology & Biotechnological Equipment1310-28181314-35302019-01-0133129430110.1080/13102818.2019.15731531573153Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterolDessislava Lazarova0Sayaka Shibata1Itsuko Ishii2Genoveva Zlateva3Zhivko Zhelev4Ichio Aoki5Rumiana Bakalova6Sofia University “St. Kliment Ohridski”National Institute of Radiological Sciences (QST-NIRS)Chiba UniversitySofia University “St. Kliment Ohridski”Trakia UniversityNational Institute of Radiological Sciences (QST-NIRS)Sofia University “St. Kliment Ohridski”We used a mitochondria-penetrating nitroxide, mito-TEMPO, as a contrast probe for imaging of kidney dysfunction in mice, based on the redox-imbalance and oxidative stress in the renal tissues. Kidney dysfunction was triggered by hypercholesterolemia. The mice were divided in three groups: (i) on normal diet (ND; control); (ii) on cholesterol diet (CD); (iii) on cholesterol plus cholestyramine diet (CC). CD mice showed increased plasma levels of total cholesterol and non-HDL-cholesterol, as well as increased serum levels of blood urea nitrogen, uric acid and creatinine, compared to ND mice. CC mice showed slightly increased plasma levels of total cholesterol and HDL-cholesterol, but not non-HDL-cholesterol, compared to ND mice. The serum levels of blood urea nitrogen, uric acid and creatinine in CC mice were equal to those in ND mice. The MRI signal of mito-TEMPO in the kidneys was characterized by: high intensity and long life-time in CD mice, indicating a high oxidative capacity of renal tissues; poor intensity and short life-time in ND mice, indicating a high reducing capacity of renal tissues; moderate intensity and relatively short life-time in CC mice, which shows the protective effect of lipid-lowering agents against oxidative damage. The data suggest that hypercholesterolemia induces redox-imbalance and oxidative stress in kidneys and this process could be visualized using MRI and mito-TEMPO as a redox-sensitive contrast substance.http://dx.doi.org/10.1080/13102818.2019.1573153hypercholesterolemiakidney dysfunctionredox-imbalanceoxidative stressmagnetic resonance imagingcyclic nitroxides |
| spellingShingle | Dessislava Lazarova Sayaka Shibata Itsuko Ishii Genoveva Zlateva Zhivko Zhelev Ichio Aoki Rumiana Bakalova Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol Biotechnology & Biotechnological Equipment hypercholesterolemia kidney dysfunction redox-imbalance oxidative stress magnetic resonance imaging cyclic nitroxides |
| title | Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol |
| title_full | Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol |
| title_fullStr | Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol |
| title_full_unstemmed | Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol |
| title_short | Imaging of redox-imbalance and oxidative stress in kidney in vivo, induced by dietary cholesterol |
| title_sort | imaging of redox imbalance and oxidative stress in kidney in vivo induced by dietary cholesterol |
| topic | hypercholesterolemia kidney dysfunction redox-imbalance oxidative stress magnetic resonance imaging cyclic nitroxides |
| url | http://dx.doi.org/10.1080/13102818.2019.1573153 |
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