Detection of Single Nucleotide Polymorphism (SNP) Variation of a Gene Sequence on Membrane-Based Lateral-Flow Strips

This study used appropriate primers to distinguish the gene model, HLA-A31:01, on membrane-based lateral-flow (MBLF) strips from its allele, which is with an SNP. Using primers designed with a mismatch base on one or two sides next to the SNP spot was verified as a good approach. In the optimal cond...

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Bibliographic Details
Main Authors: Jui-Chuang Wu, Jia-Cheng Yang, Yi-Jing Hsieh
Format: Article
Language:English
Published: MDPI AG 2022-01-01
Series:Chemosensors
Subjects:
Online Access:https://www.mdpi.com/2227-9040/10/2/59