An optimized protocol for isolation of murine pancreatic single cells with high yield and purity

An optimized protocol for isolation of murine pancreatic single cells with high yield and purity

Summary: Here, we present a protocol for rapidly isolating single cells from the mouse pancreas, minimizing damage caused by digestive enzymes in exocrine cells. We guide you through steps to optimize the dissection sequence, enzyme composition, and operational procedures, resulting in high yields o...

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Bibliographic Details
Main Authors: Feijing Wu, Zhengyu Jiang, Jin Qian, Hiroki Kobayashi, Quin T. Waterbury, Ruth A. White, Yosuke Ochiai, Xiaofei Zhi, Ruhong Tu, Biyun Zheng, Qiongyu Shi, Leah B. Zamechek, Timothy C. Wang
Format: Article
Language:English
Published: Elsevier 2024-03-01
Series:STAR Protocols
Subjects:
Cell Biology
Cell isolation
Single Cell
Flow Cytometry
Organoids
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166724000017

Internet

http://www.sciencedirect.com/science/article/pii/S2666166724000017

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