Biochemical characterization of the nuclease StoNurA from the hyperthermophilic archaeon Sulfolobus tokodaii
Abstract The DNA nuclease gene ST2109 has been cloned from the hyperthermophilic archaeon Sulfolobus tokodaii and expressed in Escherichia coli. The recombinant protein StoNurA has been purified to homogeneity by affinity chromatography and gel filtration chromatography. Biochemical analyses demonst...
Main Authors: | , , , |
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Format: | Article |
Language: | English |
Published: |
Academia Brasileira de Ciências
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Series: | Anais da Academia Brasileira de Ciências |
Subjects: | |
Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000602731&lng=en&tlng=en |