A comprehensive system for generation and evaluation of induced pluripotent stem cells using piggyBac transposition.

The most stringent criterion for evaluating pluripotency is generation of chimeric animals with germline transmission ability. Because the quality of induced pluripotent stem cell (iPSC) lines is heterogeneous, an easy and accurate system to evaluate these abilities would be useful. In this study, w...

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Bibliographic Details
Main Authors: Tomoyuki Tsukiyama, Megumi Kato-Itoh, Hiromitsu Nakauchi, Yasuhide Ohinata
Format: Article
Language:English
Published: Public Library of Science (PLoS) 2014-01-01
Series:PLoS ONE
Online Access:http://europepmc.org/articles/PMC3965495?pdf=render