Nanopore Whole Transcriptome Analysis and Pathogen Surveillance by a Novel Solid‐Phase Catalysis Approach

Nanopore Whole Transcriptome Analysis and Pathogen Surveillance by a Novel Solid‐Phase Catalysis Approach

Abstract The requirement of a large input amount (500 ng) for Nanopore direct RNA‐seq presents a major challenge for low input transcriptomic analysis and early pathogen surveillance. The high RNA input requirement is attributed to significant sample loss associated with library preparation using so...

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Bibliographic Details
Main Authors: Yi Fang, Amogh Changavi, Manyun Yang, Luo Sun, Aihua Zhang, Daniel Sun, Zhiyi Sun, Boce Zhang, Ming‐Qun Xu
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:Advanced Science
Subjects:
direct RNA‐seq
foodborne pathogen
immobilized enzymes
next‐generation sequencing
Oxford Nanopore Technologies
transcriptome
Online Access:https://doi.org/10.1002/advs.202103373

Internet

https://doi.org/10.1002/advs.202103373

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