Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A review
Abstract In recent years, the discovery and development of clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized and accelerated functional genetic screening in cancer research. In this review, we discuss different methods of executing CRISPR screens, with...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2022-12-01
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| Series: | Clinical and Translational Discovery |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/ctd2.142 |
| _version_ | 1797852213467414528 |
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| author | Madelaine G. vanMackelenbergh Dennis S. Metselaar Michaël H. Meel Esther Hulleman |
| author_facet | Madelaine G. vanMackelenbergh Dennis S. Metselaar Michaël H. Meel Esther Hulleman |
| author_sort | Madelaine G. vanMackelenbergh |
| collection | DOAJ |
| description | Abstract In recent years, the discovery and development of clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized and accelerated functional genetic screening in cancer research. In this review, we discuss different methods of executing CRISPR screens, with a focus on pediatric tumour entities. Historically, these tumours were thought to resemble their adult counterparts. However, the new era of genomic research and the extensive use of gene editing has identified pediatric tumours as distinct entities with drastically different development and presentation. Here we provide an overview of CRISPR screens performed in pediatric tumour models and highlight unique considerations for pediatric tumour screens. In particular, the results from CRISPR screens combining treatment with genetic knockouts can push treatment for pediatric patients. We conclude by discussing the potential of CRISPR genetic screening to unravel pediatric tumour biology and identify new treatment options. |
| first_indexed | 2024-04-09T19:30:19Z |
| format | Article |
| id | doaj.art-c367fff43695451181851efc3302fa01 |
| institution | Directory Open Access Journal |
| issn | 2768-0622 |
| language | English |
| last_indexed | 2024-04-09T19:30:19Z |
| publishDate | 2022-12-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Translational Discovery |
| spelling | doaj.art-c367fff43695451181851efc3302fa012023-04-05T04:47:06ZengWileyClinical and Translational Discovery2768-06222022-12-0124n/an/a10.1002/ctd2.142Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A reviewMadelaine G. vanMackelenbergh0Dennis S. Metselaar1Michaël H. Meel2Esther Hulleman3Princess Máxima Center for Pediatric Oncology Utrecht the NetherlandsPrincess Máxima Center for Pediatric Oncology Utrecht the NetherlandsPrincess Máxima Center for Pediatric Oncology Utrecht the NetherlandsPrincess Máxima Center for Pediatric Oncology Utrecht the NetherlandsAbstract In recent years, the discovery and development of clustered regularly interspaced short palindromic repeats (CRISPR) technology has revolutionized and accelerated functional genetic screening in cancer research. In this review, we discuss different methods of executing CRISPR screens, with a focus on pediatric tumour entities. Historically, these tumours were thought to resemble their adult counterparts. However, the new era of genomic research and the extensive use of gene editing has identified pediatric tumours as distinct entities with drastically different development and presentation. Here we provide an overview of CRISPR screens performed in pediatric tumour models and highlight unique considerations for pediatric tumour screens. In particular, the results from CRISPR screens combining treatment with genetic knockouts can push treatment for pediatric patients. We conclude by discussing the potential of CRISPR genetic screening to unravel pediatric tumour biology and identify new treatment options.https://doi.org/10.1002/ctd2.142CRISPRgenetic screenpediatric cancerreviewsynthetic lethality |
| spellingShingle | Madelaine G. vanMackelenbergh Dennis S. Metselaar Michaël H. Meel Esther Hulleman Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A review Clinical and Translational Discovery CRISPR genetic screen pediatric cancer review synthetic lethality |
| title | Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A review |
| title_full | Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A review |
| title_fullStr | Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A review |
| title_full_unstemmed | Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A review |
| title_short | Clustered regularly interspaced short palindromic repeats screens in pediatric tumours: A review |
| title_sort | clustered regularly interspaced short palindromic repeats screens in pediatric tumours a review |
| topic | CRISPR genetic screen pediatric cancer review synthetic lethality |
| url | https://doi.org/10.1002/ctd2.142 |
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