Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis
Objective: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. Methods: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis. Re...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2016-10-01
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| Series: | Molecular Metabolism |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S221287781630120X |
| _version_ | 1819267066767605760 |
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| author | Yen Ching Lim Sook Yoong Chia Shengnan Jin Weiping Han Chunming Ding Lei Sun |
| author_facet | Yen Ching Lim Sook Yoong Chia Shengnan Jin Weiping Han Chunming Ding Lei Sun |
| author_sort | Yen Ching Lim |
| collection | DOAJ |
| description | Objective: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. Methods: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis. Results: Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. Blocking DNA methylation with 5-Aza-cytidine increased the expression of these genes, with the most prominent effect on Hoxc10, a repressor of BAT marker expression. Conclusions: Our data suggest that DNA methylation may play an important role in lineage-specific development in adipocytes. Keywords: Brown adipogenesis, White adipogenesis, DNA methylation, Hoxc10, Next generation sequencing |
| first_indexed | 2024-12-23T21:11:16Z |
| format | Article |
| id | doaj.art-c376565141bc458b9816b22242069847 |
| institution | Directory Open Access Journal |
| issn | 2212-8778 |
| language | English |
| last_indexed | 2024-12-23T21:11:16Z |
| publishDate | 2016-10-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Molecular Metabolism |
| spelling | doaj.art-c376565141bc458b9816b222420698472022-12-21T17:31:04ZengElsevierMolecular Metabolism2212-87782016-10-0151010331041Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesisYen Ching Lim0Sook Yoong Chia1Shengnan Jin2Weiping Han3Chunming Ding4Lei Sun5School of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, SingaporeCardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, SingaporeSchool of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, ChinaSingapore Bioimaging Consortium, Agency for Science, Technology and Research (A*STAR), Singapore 138667, SingaporeSchool of Laboratory Medicine and Life Science, Wenzhou Medical University, Wenzhou, Zhejiang 325035, China; Corresponding author.Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, Singapore; Institute of Molecular and Cell Biology, 61 Biopolis Drive, Proteos, Singapore 138673, Singapore; Corresponding author. Cardiovascular and Metabolic Disorders Program, Duke-NUS Graduate Medical School, 8 College Road, Singapore 169857, Singapore.Objective: DNA methylation may be a stable epigenetic contributor to defining fat cell lineage. Methods: We performed reduced representation bisulfite sequencing (RRBS) and RNA-seq to depict a genome-wide integrative view of the DNA methylome and transcriptome during brown and white adipogenesis. Results: Our analysis demonstrated that DNA methylation is a stable epigenetic signature for brown and white cell lineage before, during, and after differentiation. We identified 31 genes whose promoters were significantly differentially methylated between white and brown adipogenesis at all three time points of differentiation. Among them, five genes belong to the Hox family; their expression levels were anti-correlated with promoter methylation, suggesting a regulatory role of DNA methylation in transcription. Blocking DNA methylation with 5-Aza-cytidine increased the expression of these genes, with the most prominent effect on Hoxc10, a repressor of BAT marker expression. Conclusions: Our data suggest that DNA methylation may play an important role in lineage-specific development in adipocytes. Keywords: Brown adipogenesis, White adipogenesis, DNA methylation, Hoxc10, Next generation sequencinghttp://www.sciencedirect.com/science/article/pii/S221287781630120X |
| spellingShingle | Yen Ching Lim Sook Yoong Chia Shengnan Jin Weiping Han Chunming Ding Lei Sun Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis Molecular Metabolism |
| title | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
| title_full | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
| title_fullStr | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
| title_full_unstemmed | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
| title_short | Dynamic DNA methylation landscape defines brown and white cell specificity during adipogenesis |
| title_sort | dynamic dna methylation landscape defines brown and white cell specificity during adipogenesis |
| url | http://www.sciencedirect.com/science/article/pii/S221287781630120X |
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