5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction
The neuroprotective role of 5-hydroxymethyl-2-furfural (5-HMF) has been demonstrated in a variety of neurological diseases. The aim of this study is to investigate the effect of 5-HMF on multiple sclerosis (MS). IFN-γ-stimulated murine microglia (BV2 cells) are considered a cell model of MS. With 5-...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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China Science Publishing & Media Ltd.
2023-07-01
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| Series: | Acta Biochimica et Biophysica Sinica |
| Subjects: | |
| Online Access: | https://www.sciengine.com/doi/10.3724/abbs.2023105 |
| _version_ | 1797635922577063936 |
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| author | Guan Dongsheng Li Yingxia Cui Yinglin Zhao Huanghong Dong Ning Wang Kun Ren Deqi Song Tiantian Wang Xiaojing Jin Shijie Gao Yinghe Wang Mengmeng |
| author_facet | Guan Dongsheng Li Yingxia Cui Yinglin Zhao Huanghong Dong Ning Wang Kun Ren Deqi Song Tiantian Wang Xiaojing Jin Shijie Gao Yinghe Wang Mengmeng |
| author_sort | Guan Dongsheng |
| collection | DOAJ |
| description | The neuroprotective role of 5-hydroxymethyl-2-furfural (5-HMF) has been demonstrated in a variety of neurological diseases. The aim of this study is to investigate the effect of 5-HMF on multiple sclerosis (MS). IFN-γ-stimulated murine microglia (BV2 cells) are considered a cell model of MS. With 5-HMF treatment, microglial M1/2 polarization and cytokine levels are detected. The interaction of 5-HMF with migration inhibitory factor (MIF) is predicted using online databases. The experimental autoimmune encephalomyelitis (EAE) mouse model is established, followed by a 5-HMF injection. The results show that 5-HMF facilitates IFN-γ-stimulated microglial M2 polarization and attenuates the inflammatory response. According to the network pharmacology and molecular docking results, 5-HMF has a binding site for MIF. Further results show that blocking MIF activity or silencing CD74 enhances microglial M2 polarization, reduces inflammatory activity, and prevents ERK1/2 phosphorylation. 5-HMF inhibits the MIF-CD74 interaction by binding to MIF, thereby inhibiting microglial M1 polarization and enhancing the anti-inflammatory response. 5-HMF ameliorates EAE, inflammation, and demyelination in vivo. In conclusion, our research indicates that 5-HMF promotes microglial M2 polarization by inhibiting the MIF-CD74 interaction, thereby attenuating inflammation and demyelination in EAE mice. |
| first_indexed | 2024-03-11T12:27:03Z |
| format | Article |
| id | doaj.art-c3767864c0244f0d9bf2aada75eead0a |
| institution | Directory Open Access Journal |
| issn | 1672-9145 |
| language | English |
| last_indexed | 2024-03-11T12:27:03Z |
| publishDate | 2023-07-01 |
| publisher | China Science Publishing & Media Ltd. |
| record_format | Article |
| series | Acta Biochimica et Biophysica Sinica |
| spelling | doaj.art-c3767864c0244f0d9bf2aada75eead0a2023-11-06T08:34:46ZengChina Science Publishing & Media Ltd.Acta Biochimica et Biophysica Sinica1672-91452023-07-01551222123310.3724/abbs.202310520d259cc5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interactionGuan Dongsheng0Li Yingxia1Cui Yinglin2Zhao Huanghong3Dong Ning4Wang Kun5Ren Deqi6Song Tiantian7Wang Xiaojing8Jin Shijie9Gao Yinghe10Wang Mengmeng11["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["The College of Basic Medicine, Henan University of Traditional Chinese Medicine, Zhengzhou 450046, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Pharmacy, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]["Department of Neurology, the Second Clinical Medical College, Henan University of Traditional Chinese Medicine, Zhengzhou 450002, China"]The neuroprotective role of 5-hydroxymethyl-2-furfural (5-HMF) has been demonstrated in a variety of neurological diseases. The aim of this study is to investigate the effect of 5-HMF on multiple sclerosis (MS). IFN-γ-stimulated murine microglia (BV2 cells) are considered a cell model of MS. With 5-HMF treatment, microglial M1/2 polarization and cytokine levels are detected. The interaction of 5-HMF with migration inhibitory factor (MIF) is predicted using online databases. The experimental autoimmune encephalomyelitis (EAE) mouse model is established, followed by a 5-HMF injection. The results show that 5-HMF facilitates IFN-γ-stimulated microglial M2 polarization and attenuates the inflammatory response. According to the network pharmacology and molecular docking results, 5-HMF has a binding site for MIF. Further results show that blocking MIF activity or silencing CD74 enhances microglial M2 polarization, reduces inflammatory activity, and prevents ERK1/2 phosphorylation. 5-HMF inhibits the MIF-CD74 interaction by binding to MIF, thereby inhibiting microglial M1 polarization and enhancing the anti-inflammatory response. 5-HMF ameliorates EAE, inflammation, and demyelination in vivo. In conclusion, our research indicates that 5-HMF promotes microglial M2 polarization by inhibiting the MIF-CD74 interaction, thereby attenuating inflammation and demyelination in EAE mice. https://www.sciengine.com/doi/10.3724/abbs.2023105multiple sclerosisexperimental autoimmune encephalomyelitis5-hydroxymethyl-2-furfuralM2 polarizationinflammation |
| spellingShingle | Guan Dongsheng Li Yingxia Cui Yinglin Zhao Huanghong Dong Ning Wang Kun Ren Deqi Song Tiantian Wang Xiaojing Jin Shijie Gao Yinghe Wang Mengmeng 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction Acta Biochimica et Biophysica Sinica multiple sclerosis experimental autoimmune encephalomyelitis 5-hydroxymethyl-2-furfural M2 polarization inflammation |
| title | 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction |
| title_full | 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction |
| title_fullStr | 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction |
| title_full_unstemmed | 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction |
| title_short | 5-HMF attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the MIF-CD74 interaction |
| title_sort | 5 hmf attenuates inflammation and demyelination in experimental autoimmune encephalomyelitis mice by inhibiting the mif cd74 interaction |
| topic | multiple sclerosis experimental autoimmune encephalomyelitis 5-hydroxymethyl-2-furfural M2 polarization inflammation |
| url | https://www.sciengine.com/doi/10.3724/abbs.2023105 |
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