A simple and inexpensive laser dissection of fasciculated axons from motor nerve organoids

A simple and inexpensive laser dissection of fasciculated axons from motor nerve organoids

Motor nerve organoids could be generated by culturing a spheroid of motor neurons differentiated from human induced pluripotent stem (iPS) cells within a polydimethylsiloxane (PDMS) chip which guides direction and fasciculation of axons extended from the spheroid. To isolate axon bundles from motor...

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Bibliographic Details
Main Authors: Yasuhiro Ikegami, Tomoya Duenki, Ikuma Arakaki, Ryo Sakai, Tatsuya Osaki, Satoshi Ashihara, Tsuyoshi Furushima, Yoshiho Ikeuchi
Format: Article
Language:English
Published: Frontiers Media S.A. 2024-01-01
Series:Frontiers in Bioengineering and Biotechnology
Subjects:
organoids
laser
tissue engineering
PDMS
axons
Online Access:https://www.frontiersin.org/articles/10.3389/fbioe.2024.1259138/full

Internet

https://www.frontiersin.org/articles/10.3389/fbioe.2024.1259138/full

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