A Novel Affinity Tag, ABTAG, and Its Application to the Affinity Screening of Single-Domain Antibodies Selected by Phage Display

A Novel Affinity Tag, ABTAG, and Its Application to the Affinity Screening of Single-Domain Antibodies Selected by Phage Display

ABTAG is a camelid single-domain antibody (sdAb) that binds to bovine serum albumin (BSA) with low picomolar affinity. In surface plasmon resonance (SPR) analyses using BSA surfaces, bound ABTAG can be completely dissociated from the BSA surfaces at low pH, over multiple cycles, without any reductio...

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Bibliographic Details
Main Authors: Greg Hussack, Toya Nath Baral, Jason Baardsnes, Henk van Faassen, Shalini Raphael, Kevin A. Henry, Jianbing Zhang, C. Roger MacKenzie
Format: Article
Language:English
Published: Frontiers Media S.A. 2017-10-01
Series:Frontiers in Immunology
Subjects:
antibody discovery
phage display
surface plasmon resonance
single-domain antibody
nanobody
VHH
Online Access:http://journal.frontiersin.org/article/10.3389/fimmu.2017.01406/full

Internet

http://journal.frontiersin.org/article/10.3389/fimmu.2017.01406/full

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