Development of a Method for Determination of brucella suis Biovars Using Multilocus Real-time PCR

Development of a Method for Determination of brucella suis Biovars Using Multilocus Real-time PCR

The aim of the study was to develop a methodological approach to determination of Brucella suis biovars through multilocus PCR with real-time registration of results.Materials and methods. We used 16 strains of B. suis of various biovars, B. neotomae and B. canis – 2 strains of each. Determination o...

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Bibliographic Details
Main Authors: N. A. Osina, D. A. Sitmbetov, I. V. Domanina, O. Yu. Lyashova, S. A. Shcherbakova, I. A. Kas’yan, Zh. A. Kas’yan, E. G. Bulgakova
Format: Article
Language:Russian
Published: Federal Government Health Institution, Russian Research Anti-Plague Institute “Microbe” 2022-07-01
Series:Проблемы особо опасных инфекций
Subjects:
multilocus pcr
intraspecific differentiation
brucella suis
biovars
incp and gi-3 genomic islands
structural organization
sanger fragment sequencing
Online Access:https://journal.microbe.ru/jour/article/view/1702

Internet

https://journal.microbe.ru/jour/article/view/1702

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