Quantitative Detection of Viable but Nonculturable <i>Cronobacter sakazakii</i> Using Photosensitive Nucleic Acid Dye PMA Combined with Isothermal Amplification LAMP in Raw Milk

Quantitative Detection of Viable but Nonculturable <i>Cronobacter sakazakii</i> Using Photosensitive Nucleic Acid Dye PMA Combined with Isothermal Amplification LAMP in Raw Milk

An accurate method that rapidly detects the number of viable but nonculturable (VBNC) <i>Cronobacter sakazakii</i> was developed by combining propidium bromide with quantitative LAMP (PMA-QLAMP). The <i>gyrB</i> gene was the target for primers design. The optimal PMA treatmen...

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Bibliographic Details
Main Authors: Lianxia Hu, Shufei Zhang, Yuling Xue, Yaoguang Zhang, Wei Zhang, Shijie Wang
Format: Article
Language:English
Published: MDPI AG 2022-09-01
Series:Foods
Subjects:
viable but nonculturable
<i>Cronobacter sakazakii</i>
propidium bromide
quantitative loop-mediated isothermal amplification
raw milk
pasteurization
Online Access:https://www.mdpi.com/2304-8158/11/17/2653

Internet

https://www.mdpi.com/2304-8158/11/17/2653

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