Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography
A selective method for the quantitation of pantoprazole and its metabolite in goat plasma was developed and validated. Chloroform was used for drug extraction and separation occurred on a Symmetry C18 column. A combination of sodium phosphate dibasic (0.1 M, pH 7.5) and acetonitrile were used as the...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Elsevier
2022-11-01
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| Series: | Journal of Chromatography Open |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2772391722000093 |
| _version_ | 1811187607659347968 |
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| author | Sherry Cox Lainey Harvill Sarah Bullock Joe Smith Joan Bergman |
| author_facet | Sherry Cox Lainey Harvill Sarah Bullock Joe Smith Joan Bergman |
| author_sort | Sherry Cox |
| collection | DOAJ |
| description | A selective method for the quantitation of pantoprazole and its metabolite in goat plasma was developed and validated. Chloroform was used for drug extraction and separation occurred on a Symmetry C18 column. A combination of sodium phosphate dibasic (0.1 M, pH 7.5) and acetonitrile were used as the mobile phase (64:36, v/v) while absorbance was measured at 290 nm. With a sample size of 0.1 mL the lower limit of quantification (LLOQ) was 0.01 µg/mL. The intra-assay variability for pantoprazole ranged from 3.4 to 10% while the metabolite ranged from 3.3% to 8.7% The inter-assay variability ranged from 2.6 to 9.7% and 3.3 to 7.5% for pantoprazole and its metabolite. The recovery was over 95% for both pantoprazole and its metabolite, pantoprazole sulfone. The method was used to quantify both pantoprazole and its metabolite and was useful for pharmacokinetic studies. |
| first_indexed | 2024-04-11T14:06:15Z |
| format | Article |
| id | doaj.art-c3de7dcfe10e494ab7e7069fdbcee476 |
| institution | Directory Open Access Journal |
| issn | 2772-3917 |
| language | English |
| last_indexed | 2024-04-11T14:06:15Z |
| publishDate | 2022-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Journal of Chromatography Open |
| spelling | doaj.art-c3de7dcfe10e494ab7e7069fdbcee4762022-12-22T04:19:53ZengElsevierJournal of Chromatography Open2772-39172022-11-012100038Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatographySherry Cox0Lainey Harvill1Sarah Bullock2Joe Smith3Joan Bergman4Department of Biomedical and Diagnostic Sciences, The University of Tennessee Knoxville College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USA; Corresponding author.Department of Biomedical and Diagnostic Sciences, The University of Tennessee Knoxville College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USADepartment of Biomedical and Diagnostic Sciences, The University of Tennessee Knoxville College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USADepartment of Large Animal Clinical Sciences, The University of Tennessee, College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USADepartment of Biomedical and Diagnostic Sciences, The University of Tennessee Knoxville College of Veterinary Medicine, 2407 River Drive, Knoxville, TN 37996, USAA selective method for the quantitation of pantoprazole and its metabolite in goat plasma was developed and validated. Chloroform was used for drug extraction and separation occurred on a Symmetry C18 column. A combination of sodium phosphate dibasic (0.1 M, pH 7.5) and acetonitrile were used as the mobile phase (64:36, v/v) while absorbance was measured at 290 nm. With a sample size of 0.1 mL the lower limit of quantification (LLOQ) was 0.01 µg/mL. The intra-assay variability for pantoprazole ranged from 3.4 to 10% while the metabolite ranged from 3.3% to 8.7% The inter-assay variability ranged from 2.6 to 9.7% and 3.3 to 7.5% for pantoprazole and its metabolite. The recovery was over 95% for both pantoprazole and its metabolite, pantoprazole sulfone. The method was used to quantify both pantoprazole and its metabolite and was useful for pharmacokinetic studies.http://www.sciencedirect.com/science/article/pii/S2772391722000093PantoprazolePlasmaPharmacokinetic studiesUV detection |
| spellingShingle | Sherry Cox Lainey Harvill Sarah Bullock Joe Smith Joan Bergman Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography Journal of Chromatography Open Pantoprazole Plasma Pharmacokinetic studies UV detection |
| title | Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography |
| title_full | Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography |
| title_fullStr | Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography |
| title_full_unstemmed | Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography |
| title_short | Validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography |
| title_sort | validation of a method for pantoprazole and its sulfone metabolite in goat plasma using high performance liquid chromatography |
| topic | Pantoprazole Plasma Pharmacokinetic studies UV detection |
| url | http://www.sciencedirect.com/science/article/pii/S2772391722000093 |
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