Cholera Toxin Subunit B for Sensitive and Rapid Determination of Exosomes by Gel Filtration

We developed a sensitive fluorescence-based assay for determination of exosome concentration. In our assay, Cholera toxin subunit B (CTB) conjugated to a fluorescence probe and a gel filtration technique (size-exclusion chromatography) are used. Exosomal membranes are particularly enriched in raft-f...

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Bibliographic Details
Main Authors: Karolina Sapoń, Dominika Maziarz, Teresa Janas, Aleksander F. Sikorski, Tadeusz Janas
Format: Article
Language:English
Published: MDPI AG 2020-07-01
Series:Membranes
Subjects:
Online Access:https://www.mdpi.com/2077-0375/10/8/172