High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in

High-quality single amplicon sequencing method for illumina MiSeq platform using pool of ‘N’ (0–10) spacer-linked target specific primers without PhiX spike-in

Abstract Background Illumina sequencing platform requires base diversity in the initial 11 cycles for efficient cluster identification and colour matrix estimation. This limitation yields low-quality data for amplicon libraries having homogeneous base composition. Spike-in of PhiX library ensures ba...

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Bibliographic Details
Main Authors: Tejali Naik, Mohak Sharda, Lakshminarayanan C P, Kumar Virbhadra, Awadhesh Pandit
Format: Article
Language:English
Published: BMC 2023-03-01
Series:BMC Genomics
Subjects:
Amplicon sequencing
‘N’ (0–10) spacer-linked primer pool
Illumina
PhiX spike-in
Online Access:https://doi.org/10.1186/s12864-023-09233-4

Internet

https://doi.org/10.1186/s12864-023-09233-4

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